Mouse CD3+ T Cell, Small Column Enrichment Kit Summary
| Description |
Mononuclear cell suspensions are loaded onto T Cell Enrichment Columns containing glass beads coated with Ig and anti-Ig. B cells bind to anti-Ig coated beads by F(ab)-surface Immunoglobulin (Ig) interactions. Monocytes bind to Ig coated beads via Fc interactions. The resulting column eluate contains highly enriched T cell populations. CD3+ is a universal marker for T cells. Total CD3+ cell recovery ranges between 45% and 65% of initial CD3+ cells loaded. The purity of recovered cells ranges from 81% to 88%. These enriched T cell populations are then available for cell culture, activation studies, phenotyping, immune status monitoring, and flow cytometry. Designed to prepare purified murine T cell populations via high affinity negative selection. The resulting column eluate is a highly enriched T cell population with minimal depletion of T cell constituents. Key Benefits: Available for human, mouse, and rat cellsPurifies CD3+ T cells to 81 - 88%Based upon gravitational flowSimple procedureNo specialized equipment required |
| Kit Type |
Enrichment Kit |
| Gene |
CD3 |
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze. |
Kit Components
|
Components
|
- Murine T Cell Selection Columns, 10X Column Buffer Concentrate
|
Background
The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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