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Lightning-Link® Rapid Atto488 Antibody Labeling Kit

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Flow Cytometry: Lightning-Link Rapid Atto488 Antibody Labeling Kit [350-0010] - Mouse anti-human CD3 was conjugated with Atto488 using Lightning-Link® Rapid kit. The conjugated antibody was then used to stain human ...read more

Product Details

Summary
Conjugate
Atto488
Format
Atto488 (A=501, E=523)
Kit Type
Antibody Labeling Kit

Order Details

  • Size / Catalog#QtyPrice
    350-0010
    3 x 100ug Reaction
    $629.00
    6-8 Business Days
Shipping $45.00 per order
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Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

Lightning-Link® Rapid Atto488 Antibody Labeling Kit Summary

Description
Lightning-Link Rapid is an innovative technology that enables direct labeling of proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information).

The easy-to-use, one step procedure allows researchers to covalently label biomolecules with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.

The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates (for more details please watch the video below).

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Atto488 is one of a new generation of fluorescent labels, which has been optimised for excitation with an argon laser. It has a strong absorption at 504nm, high fluorescence at 530nm (extinction coefficient 9.0 x104 cm-1M-1) and high quantum yield.

Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay

Kit Type
Antibody Labeling Kit

Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%.
Publications
Read Publications using 350-0010.

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. 1 or 3 glass vial(s) of Lightning-Link Rapid mix
  2. 1 vial of LL-Rapid Modifier reagent
  3. 1 vial of LL-Rapid Quencher reagent

Notes

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

Background

Lightning-Link® Rapid Antibody Labeling kits

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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