| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IP, CyTOF-ready |
| Clone | MEM-09 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Janelia Fluor 646 |
| Immunogen | Crude thymus membrane fraction. |
| Specificity | The antibody MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with kappa light chains (22.5 kDa). Material immunoprecipitated from human serum with the antibody MEM-09 consists of IgG and traces of IgM. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | IGKC |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Kappa Light Chain Antibody (NB500-331JF646)Find related products by research area.
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| Gene Symbol | IGKC |
![Immunocytochemistry/Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463] - JJN3 and 8226 Human myeloma cells stained with NB7463. Cells fixed in 4%PFA 10min, wash PBS X 3. Permeabilized 0.5% Triton X-100 5min; wash PBS X 3. Block 10% donkey serum 1hr. 1:10,000 NB7463 O/N at 4C; wash PBS X 3 and 1:2000 donkey F(ab')2 Alexa Fluor Cy3 anti-goat IgG (H+L) 1 hour at room temp; wash PBS X 3. Olympus/BIOVIEW Cy3 filter 300ms exposure. Image form verified customer review.](https://images.novusbio.com/images/Goat-anti-Human-Kappa-Light-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB7463-img0001.jpg "Immunocytochemistry/Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463] - JJN3 and 8226 Human myeloma cells stained with NB7463. Cells fixed in 4%PFA 10min, wash PBS X 3. Permeabilized 0.5% Triton X-100 5min; wash PBS X 3. Block 10% donkey serum 1hr. 1:10,000 NB7463 O/N at 4C; wash PBS X 3 and 1:2000 donkey F(ab')2 Alexa Fluor Cy3 anti-goat IgG (H+L) 1 hour at room temp; wash PBS X 3. Olympus/BIOVIEW Cy3 filter 300ms exposure. Image form verified customer review.")