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Intracellular Staining Flow Cytometry Kit Summary
Description
This kit is optimized for intracellular staining of cells in flow cytometric applications. It is designed and optimized to minimize non-specific staining while maximizing signal-to-noise ratio for clear and consistent data.
Kit Type
Flow Cytometry Kit
Applications/Dilutions
Dilutions
Flow (Intracellular)
Flow Cytometry
Application Notes
Brefeldin A is an inhibitor of intracellular protein transport. It blocks translocation of proteins from the endoplasmic reticulum to the Golgi apparatus and can enhance detection of some intracellular proteins. Researchers are encouraged to determine whether or not Brefeldin A is needed to assay for the protein of interest. Brefeldin A is light-sensitive, store at 4C in the dark. It should be diluted in culture media (1:1000) just prior to use. Add to cell culture and incubate for 4hr before harvesting cells. Use in Flow Intracellular reported in scientific literature (PMID 24804954).
Publications
Read Publications using NBP2-29450 in the following applications:
Human reactivity reported in scientific literature (PMID: 24804954).
Packaging, Storage & Formulations
Storage
Storage of components varies. See protocol for specific instructions.
Kit Components
Components
1000X Brefeldin A (100 uL)
10X Permeabilization Buffer (2 x 60 mL)
1X Fixation Buffer (60 mL)
1X Staining Buffer (2 x 60 mL)
Alternate Names for Intracellular Staining Flow Cytometry Kit
Permeabilization buffer
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
Publications for Intracellular Staining Kit (NBP2-29450)(6)
We have publications tested in 2 confirmed species: Human, Canine.
We have publications tested in 4 applications: FLOW, Flow Cytometry Control, ICC/IF, WB.
Reviews for Intracellular Staining Kit (NBP2-29450) (0)
There are no reviews for Intracellular Staining Kit (NBP2-29450).
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Product General Protocols
View specific protocols for Intracellular Staining Kit (NBP2-29450):
FAQs for Intracellular Staining Kit (NBP2-29450). (Showing 1 - 1 of 1 FAQs).
Does your Intracellular Toll-like Receptor Staining Flow Assay Kit [NBP2-29450] contain Sodium Azide (NAN3)? If yes, how much NAN3 does it have?
Yes, there is Sodium Azide in the permeablization and staining buffers in this kit. Permeablization and staining buffers contain 0.05 % sodium azide. Use caution when handling. All the materials included in this kit should be treated as hazardous materials and be disposed of accordingly.
![Flow Cytometry: Intracellular Staining Flow Cytometry Kit [NBP2-29450] - Analysis of intracellular TLR9 in Ramos cells using IC-Flow kit to fix and permeabilize cells. Blue: Unstained Ramos cells, Green: Isotype control at 2 ug /10^6 Ramos cells, Red: Anti-TLR9 at 2 ug /10^6 Ramos cells.](http://images.novusbio.com/fullsize/Intracellular-Toll-like-Receptor-Staining-Flow-Assay-Kit-Flow-Cytometry-NBP2-29450-img0001.jpg "Flow Cytometry: Intracellular Staining Flow Cytometry Kit [NBP2-29450] - Analysis of intracellular TLR9 in Ramos cells using IC-Flow kit to fix and permeabilize cells. Blue: Unstained Ramos cells, Green: Isotype control at 2 ug /10^6 Ramos cells, Red: Anti-TLR9 at 2 ug /10^6 Ramos cells.")
