| Reactivity | MuSpecies Glossary |
| Applications | ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | FITC |
| Concentration | LYOPH |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-69562 | |||
| HRP | NBP2-69563 | |||
| TRITC | NBP2-69564 | |||
| Unconjugated | NBP2-69561 | |||
| Description | The lyophilized conjugate is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. |
| Immunogen | Pools of purified homogenous IgG2a isolated from mouse serum of strains belonging to the allelic types Igh-1a an Igh-1b. Freund's complete adjuvant is used in the first step of the immunization procedure. |
| Specificity | The reactivity of the antiserum is directed to the subclass IgG2a as tested against mouse sera and a panel of purified homogenous immunoglobulins. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Further tested on the level of sensitivity of direct and indirect immunofluorescence staining methods applied to lymphoid cells. When used on vital peripheral blood cells this immunoconjugate does not react with Fc receptors present on cell surface membranes. |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Ion exchange chromatography |
| Dilutions |
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| Application Notes | (In)direct immunofluorescence |
| Storage | Store at 4C in the dark. |
| Buffer | Lyophilized from PBS (pH 7.2) |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Ion exchange chromatography |
| Reconstitution Instructions | Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C. |
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