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Human IgG1 ELISA Kit (Chemiluminescence)

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ELISA: Human IgG1 ELISA Kit (Chemiluminescence) [NBP3-00401] - Samples were spiked with high concentrations of Human IgG1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human IgG1 ELISA Kit (Chemiluminescence) [NBP3-00401] - Samples were spiked with high concentrations of Human IgG1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Human IgG1 ELISA Kit (Chemiluminescence) [NBP3-00401] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.31 - 20 ug/mL
Sensitivity
0.19 ug/mL

Order Details

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Human IgG1 ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Human IgG1 in samples. No significant cross-reactivity or interference between Human IgG1 and analogues was observed.
Standard Curve Range
0.31 - 20 ug/mL
Sensitivity
0.19 ug/mL
Assay Type
Sandwich-ELISA
Inter-Assay
4.6%
Intra-Assay
4.68%
Spike Recovery
86 - 106%
Sample Volume
100 ul
Kit Type
ELISA Kit (Chemiluminescence)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Human IgG1 ELISA Kit (Chemiluminescence)

  • IgG1
  • Igh-4
  • RGD1359539
  • VH7183

Background

IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Product PDFs

  Human IgG1 ELISA Kit (Chemiluminescence) Manual
  PDF Datasheet
  Request CofA

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