| Reactivity | MuSpecies Glossary |
| Applications | WB, DB, ELISA, ICC/IF, WB |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Description | The lyophilized IgG (7S) fraction is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. |
| Immunogen | Pools of purified homogenous IgG1 isolated from pooled mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure. |
| Specificity | The reactivity of the antiserum is directed to the subclass IgG1 as tested against mouse sera and a panel of purified homogenous immunoglobulins. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Ion exchange chromatography |
| Dilutions |
|
| Application Notes | Indirect immunofluorescence |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.2) |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Ion exchange chromatography |
| Reconstitution Instructions | Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C. |
Research Areas for IgG1 Antibody (NBP2-69518)Find related products by research area.
|
|
Read full blog post. |
|
CD16 - Find me on macrophages, neutrophils and NK cells CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells.... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
