Rabbit anti-Mouse IgG (H+L) Secondary Antibody

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 6 applications: Cytometric Bead Assay Standard, ELISA, ICC/IF, IF/IHC, IHC-P, WB.

Showing Publications 1 - 10 of 11. Show All 11 Publications. Collapse Publications.

Publications using NB7541	Applications	Species
Atzori MG, Ceci C, Ruffini F Et al. The Anti-Vascular Endothelial Growth Factor Receptor 1 (VEGFR-1) D16F7 Monoclonal Antibody Inhibits Melanoma Adhesion to Soluble VEGFR-1 and Tissue Invasion in Response to Placenta Growth Factor Cancers (Basel) 2022-11-26 [PMID: 36428669] (IHC-P) Details: Citation using the HRP version of this antibody.	IHC-P
Ji Y, Liang Y, Chu PH Et al. The effects of intermittent hypoxia on hepatic expression of fatty acid translocase CD36 in lean and diet-induced obese mice Biomed J 2022-10-16 [PMID: 36244649] (WB) Details: Citation using the HRP version of this antibody.	WB
van der Woude R, Turner HL, Tomris I et al. Drivers of recombinant soluble influenza A virus hemagglutinin and neuraminidase expression in mammalian cells Protein Sci. 2020-07-25 [PMID: 32710576] (ELISA)	ELISA
Gunasekar P, Satish M, Dabestani P et al. Modulation of Cathepsin L Expression in the Coronary Arteries of Atherosclerotic Swine J Surg Res 2019-08-05 [PMID: 31377485] (WB)	WB
Qureshi H, Hamid SS, Ali SS et al. Cytotoxic effects of aflatoxin B1 on human brain microvascular endothelial cells of the blood-brain barrier Med. Mycol. [PMID: 25851265] (Cytometric Bead Assay Standard) Details: Citation using the HRP form of this antibody.	Cytometric Bead Assay Standard
Allen JL, Mellor H. The coculture organotypic assay of angiogenesis. Methods Mol Biol. [PMID: 25468611] (ICC/IF) Details: Citation using the Alkaline Phosphatase form of this antibody.	ICC/IF
Freitas-Ribeiro GM, Fonseca CC, Sartori SS et al. Endocrine cells and nerve ganglia of the small intestine of the Opossum Didelphis aurita Wied-Neuwied, 1826 (Mammalia: Didelphidae). An Acad Bras Cienc. [PMID: 22801379] Details: Citation using the HRP form of this antibody.
Novaes RD, Goncalves RV, Penitente AR et al. Trypanosoma cruzi infection alters glucose metabolism at rest and during exercise without modifying the morphology of pancreatic islets in rats. Pathol Res Pract. [PMID: 22749578] Details: Citation using the HRP form of this antibody.
Basile DR, Novaes RD, Marques DC et al. Analysis of the morphology and distribution of argentaffin, argyrophil and insulin-immunoreactive endocrine cells in the small intestine of the adult opossum Didelphis aurita (Wied-Neuwied, 1826). Tissue Cell. [PMID: 22608000] Details: Citation using the HRP form of this antibody.
Janssen L, Saranya M, Leinonen M et al. Vertically aligned carbon nanotube micropillars induce unidirectional chondrocyte orientation Carbon 2019-11-01 (IF/IHC)	IF/IHC
Bullock JJ, Mehta SL, Lin Y et al. Hyperglycemia-enhanced ischemic brain damage in mutant manganese SOD mice is associated with suppression of HIF-1alpha Neurosci Lett 2009-05-12 [PMID: 19429140]
Show All 11 Publications. Collapse Publications.

Array NB7541

• IgG (H+L) Antibodies