![Dot Blot: Mouse IgG Fc Isotype Control [Biotin] [NBP1-97293]](http://images.novusbio.com/fullsize/Mouse-IgG-Fc-Isotype-Control-[Biotin]-Dot-Blot-NBP1-97293-img0002.jpg "Dot Blot: Mouse IgG Fc Isotype Control [Biotin] [NBP1-97293]")
| Reactivity | MuSpecies Glossary |
| Applications | WB, DB, ELISA, PAGE |
| Host | Mouse |
| Conjugate | Biotin |
| Concentration | LYOPH |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NBP1-97278 | |||
| HRP | NBP1-97299 | |||
| Unconjugated | NBP1-96784 | |||
| Description | Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Mouse IgG, anti-Mouse IgG F(c) and anti-Mouse Serum |
| Specificity | No reaction was observed against anti-Mouse IgG F(ab')2 or anti-Papain. |
| Isotype | IgG |
| Host | Mouse |
| Purity | Multi-step |
| Dilutions |
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| Application Notes | This product has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. |
| Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Sodium Azide |
| Concentration | LYOPH |
| Purity | Multi-step |
| Reconstitution Instructions | Reconstitute with 1.0 ml deionized water (or equivalent). |
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![SDS-Page: Mouse IgG Fc Isotype Control [Biotin] [NBP1-97293] - SDS-PAGE of Mouse IgG Fc Isotype control [Biotin]. Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment Biotin Conjugated. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Kappa Myeloma Protein. Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.](http://images.novusbio.com/fullsize/Mouse-IgG-Fc-Isotype-Control-[Biotin]-SDS-Page-NBP1-97293-img0003.jpg "SDS-Page: Mouse IgG Fc Isotype Control [Biotin] [NBP1-97293] - SDS-PAGE of Mouse IgG Fc Isotype control [Biotin]. Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment Biotin Conjugated. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Kappa Myeloma Protein. Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.")
![Western Blot: Goat anti-Human IgG Fc Secondary Antibody [NB7446] - Image from verified customer review. Image using the HRP form of this antibody.](https://images.novusbio.com/images/Goat-anti-Human-IgG-Fc-Secondary-Antibody-Western-Blot-NB7446-img0001.jpg "Western Blot: Goat anti-Human IgG Fc Secondary Antibody [NB7446] - Image from verified customer review. Image using the HRP form of this antibody.")