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Goat anti-Human IgG Fc Secondary Antibody [HRP] (Pre-adsorbed)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Format
Pre-adsorbed
Concentration
LYOPH

Goat anti-Human IgG Fc Secondary Antibody [HRP] (Pre-adsorbed) Summary

Description
Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.
Immunogen
Purified Human IgG, Fc fragment
Specificity
Based on IEP, this antibody reacts with heavy gamma chains on human IgG. This antibody has been pre-adsorbed against human IgA + IgM.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:200-1:5000
  • Immunohistochemistry 1:500-1:5000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200-1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.
Readout System

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin human serum immunoglobulins, light chains on all human immunoglobulins, and human IgA or IgM

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.1% Kathon
Concentration
LYOPH
Purity
Affinity purified
Reconstitution Instructions
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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