Goat anti-Human IgG Fc Secondary Antibody [Janelia Fluor 646] (Pre-adsorbed)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Janelia Fluor 646
Format
Pre-adsorbed

Goat anti-Human IgG Fc Secondary Antibody [Janelia Fluor 646] (Pre-adsorbed) Summary

Immunogen
Purified Human IgG, Fc fragment
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on human IgG. Cross Adsorbed: This antibody has been pre-adsorbed against mouse IgG1 and bovine, horse, mouse, and rabbit serum proteins to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with 0 IgG serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Based on IEP, this antibody reacts with heavy (gamma) chains on human IgG.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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