| Reactivity | ShSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Alkaline Phosphatase |
| Format | Pre-adsorbed |
| Description | Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum |
| Immunogen | Sheep IgG F(c) fragment |
| Specificity | No reaction was observed against Sheep IgG F(ab) or Human Serum Proteins.. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Multi-step |
| Dilutions |
|
| Application Notes | This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:14,000 of the reconstitution concentration is suggested for this product. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Sodium Azide |
| Purity | Multi-step |
Research Areas for Sheep IgG, Fc Antibody (NBP1-72690)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
