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Mouse IgG F(ab)2 Isotype Control [Biotin]

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SDS-Page: Mouse IgG F(ab) Isotype Control [Biotin] [NBP1-97198] - Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab')2 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications PAGE
Host
Mouse
Conjugate
Biotin
Format
Azide and BSA Free
Concentration
LYOPH

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Mouse IgG F(ab)2 Isotype Control [Biotin] Summary

Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Mouse IgG, anti-Mouse IgG F(ab')2 and anti-Mouse Serum
Specificity
No reaction was observed against anti-Mouse IgG F(c) or anti-Pepsin.
Isotype
IgG
Host
Mouse
Purity
Multi-step

Applications/Dilutions

Dilutions
  • SDS-Page 1:100 - 1:2000
Application Notes
This product can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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