Reactivity | DoSpecies Glossary |
Applications | WB, ELISA, Flow, IHC |
Host | Donkey |
Conjugate | Unconjugated |
Format | Purified |
Concentration | LYOPH |
Description | This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey IgG, anti-Donkey IgG F(ab')2 and anti-Donkey Serum Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
Specificity | No reaction was observed against anti-Donkey IgG F(c) or anti-Pepsin. |
Isotype | IgG |
Host | Donkey |
Purity | Multi-step |
Dilutions |
|
Application Notes | This product has been tested by SDS-Page and are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods. |
Storage | Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Preservative | 0.01% Sodium Azide |
Concentration | LYOPH |
Purity | Multi-step |
Reconstitution Instructions | Reconstitute with 1.0 ml deionized water (or equivalent). |
Research Areas for IgG F(ab')2 Isotype Control (NBP1-97009)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.