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Goat anti-Human IgE Heavy Chain Secondary Antibody [HRP]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
LYOPH

Goat anti-Human IgE Heavy Chain Secondary Antibody [HRP] Summary

Description
Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.

Store lyophilized material at 2-8C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20C as a liquid.
Immunogen
Purified human IgE, (Epsilon chain)
Specificity
Based on IEP, this antibody reacts with heavy epsilon chains on human IgE
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:200-1:5000
  • Immunohistochemistry 1:500-1:5000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200-1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.
Readout System

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin human serum immunoglobulins and light chains on all human immunoglobulins

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.1% Kathon
Concentration
LYOPH
Purity
Affinity purified
Reconstitution Instructions
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Alternate Names for Goat anti-Human IgE Heavy Chain Secondary Antibody [HRP]

  • Constant Region of Heavy Chain of IgE
  • Ig Epsilon Chain C Region ND
  • Ig Epsilon Chain C Region
  • IgE
  • Immunoglobulin Epsilon
  • immunoglobulin heavy constant epsilon

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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WB Video Protocol

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