![ELISA: Goat anti-Canine IgE Heavy Chain Secondary Antibody [NB7343] - Sandwich ELISA for total dog IgE measurement showing standard curve at 2 different coating antibody concentrations. Coating: 1ug/ml in carbonate buffer, pH 9.6. Standard: purified dog IgE, range: 156pg/ml to 15.000 pg/ml. Detection with secondary antibody (HRP-labeled) and TMB substrate. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Goat-anti-Canine-IgE-Secondary-Antibody-ELISA-NB7343-img0001.jpg "ELISA: Goat anti-Canine IgE Heavy Chain Secondary Antibody [NB7343] - Sandwich ELISA for total dog IgE measurement showing standard curve at 2 different coating antibody concentrations. Coating: 1ug/ml in carbonate buffer, pH 9.6. Standard: purified dog IgE, range: 156pg/ml to 15.000 pg/ml. Detection with secondary antibody (HRP-labeled) and TMB substrate. This image was submitted via customer Review.")
| Reactivity | CaSpecies Glossary |
| Applications | WB, DB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7343APC | |||
| Biotin | NB7343B | |||
| DyLight 350 | NB7343UV | |||
| DyLight 405 | NB7343V | |||
| DyLight 488 | NB7343G | |||
| DyLight 550 | NB7343R | |||
| DyLight 594 | NB7343DL594 | |||
| DyLight 650 | NB7343C | |||
| DyLight 680 | NB7343FR | |||
| DyLight 755 | NB7343IR | |||
| FITC | NB7345 | |||
| HRP | NB7346 | |||
| PE | NB7343PE | |||
| PerCP | NB7343PCP | |||
| Immunogen | Dog IgE |
| Specificity | By immunoelectrophoresis and ELISA this reacts specifically with dog IgE. This may cross react with IgE from other species. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Immunogen affinity purified |
| Dilutions |
|
|
| Application Notes | ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500 |
|
| Reviewed Applications |
|
|
| Publications |
|
| Storage | Store at 4C. Do not freeze. |
| Buffer | Phosphate Buffered Saline (PBS) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
reviewed by:
Verified Customer |
ELISA | Canine | 02/05/2019 |
Summary
|
|||||||||
Enlarge |
reviewed by:
Verified Customer |
01/31/2017 |
Summary
Comments
|
Research Areas for IgE Heavy Chain Antibody (NB7343)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
5 | |
4 | |
3 | |
2 | |
1 |
| Verified Customer 02/05/2019 |
||
| Application: | ELISA | |
| Species: | Canine |
| Verified Customer 01/31/2017 |
||
| Application: | ||
| Species: |
![Dot Blot: Goat anti-Canine IgE Secondary Antibody [NB7343] - D.p. extracts have effects on MCs though the IgE-FcepsilonRI axis. SDS-PAGE of recombinants (300 ng) and Western blotting (168.8 ng, for rDer p 15; 143.8 ng of rDer p 18 per gel lane) of specific IgE in canine sera. Image collected and cropped by CiteAb from the following publication (//waojournal.biomedcentral.com/articles/10.1186/s40413-017-0154-3), licensed under a CC-BY license.. Using the HRP format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Canine-IgE-Secondary-Antibody-Dot-Blot-NB7343-img0002.jpg "Dot Blot: Goat anti-Canine IgE Secondary Antibody [NB7343] - D.p. extracts have effects on MCs though the IgE-FcepsilonRI axis. SDS-PAGE of recombinants (300 ng) and Western blotting (168.8 ng, for rDer p 15; 143.8 ng of rDer p 18 per gel lane) of specific IgE in canine sera. Image collected and cropped by CiteAb from the following publication (//waojournal.biomedcentral.com/articles/10.1186/s40413-017-0154-3), licensed under a CC-BY license.. Using the HRP format of this antibody.")
