Recombinant HSP65 (mycobacterial) Protein Summary
| Description |
Recombinant protein for Bacteria HSP65 (mycobacterial) Source:E. coli
Amino Acid Sequence: (P0A520) |
Preparation
Method |
This protein was expressed in E. coli and multi-step purified. |
| Source |
E. coli |
| Protein/Peptide Type |
Recombinant Protein |
| Gene |
groEL2 |
| Purity |
>90%, by SDS-PAGE |
Applications/Dilutions
| Dilutions |
- ELISA
- SDS-Page
- Western Blot
|
| Theoretical MW |
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer |
10 mM Tris (pH 8), 105 mM NaCl, 5 mM EDTA |
| Purity |
>90%, by SDS-PAGE |
Alternate Names for Recombinant HSP65 (mycobacterial) Protein
Background
HSP65 isolated from Mycobacterium bovis BCG, is a member of the HSP60 family of heat shock proteins. HSP60s are mitochondrial chaperonins that are typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. In addition to its role as a heat shock protein, HSP60 functions as a chaperonin to assist in folding linear amino acid chains into their respective three-dimensional structure. HSP60s are a ubiquitous class of HSPs that specifically promote the folding and assembly of cellular polypeptides in an ATP-dependent manner. Specifically, sequence comparison of HSP65 from different mycobacterium strains showed that the protein sequence of M. bovis BCG is identical to that of M. tuberculosis, and very similar to that of M. leprae, the pathogens that cause tuberculosis and tuberculoid leprosy, respectively. Mycobacterium bovis BCG HSP65 was identified as the immunodominant antigen during mycobacterial diseases and vaccination. It is also believed to be the antigen that induces autoimmune disease, such as adjuvant arthritis in rats.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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Blogs on HSP65 (mycobacterial)
![SDS-Page: Recombinant HSP65 (mycobacterial) Protein [NBP3-09170] - SDS-PAGE of 65kDa M. Bovis Hsp65 protein (NBP3-09170).](http://images.novusbio.com/fullsize/Recombinant-HSP65-mycobacterial-Protein-SDS-Page-NBP3-09170-img0001.jpg "SDS-Page: Recombinant HSP65 (mycobacterial) Protein [NBP3-09170] - SDS-PAGE of 65kDa M. Bovis Hsp65 protein (NBP3-09170).")
