Recombinant Human Glutathione S-transferase Mu 5 His Protein Summary
Description |
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-218 of Human Glutathione S-transferase Mu 5 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMPMTLG YWDIRGLAHA IRLLLEYTDS SYVEKKYTLG DAPDYDRSQW LNEKFKLGLD FPNLPYLIDG AHKITQSNAI LRYIARKHNL CGETEEEKIR VDILENQVMD NHMELVRLCY DPDFEKLKPK YLEELPEKLK LYSEFLGKRP WFAGDKITFV DFLAYDVLDM KRIFEPKCLD AFLNLKDFIS RFEGLKKISA YMKSSQFLRG LLFGKSATWN SK |
Details of Functionality |
Specific activity is > 90,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C. |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
GSTM5 |
Purity |
>95%, by SDS-PAGE |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
28.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol, 0.1 M NaCl |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
>95%, by SDS-PAGE |
Alternate Names for Recombinant Human Glutathione S-transferase Mu 5 His Protein
Background
The GSTM5 (glutathione S-transferase Mu 5) gene codes for a 218 amino acid long, 25 kDA protein that functions as a catalyst in various reactions in eukaryotes and prokaryotes such as converting reduced gluthathione to a large number of exogenous and endogenous hydrophobic electrophiles. GSTM5 has been linked to various diseases such as hypertension, hepatitis, adenocarcinoma, breast cancer, coronary heart disease, and hypertension. GSTM5 participates in gluthione metabolism and conjugation as well as metapathway biotransformation and has been known to interact with various genes such as CYP1A1, CYP1B1, CYP2B6, CYP2C19, and ALDH1A3.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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