Glutathione S-transferase Mu 5 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

The GSTM5 (glutathione S-transferase Mu 5) gene codes for a 218 amino acid long, 25 kDA protein that functions as a catalyst in various reactions in eukaryotes and prokaryotes such as converting reduced gluthathione to a large number of exogenous and endogenous hydrophobic electrophiles. GSTM5 has been linked to various diseases such as hypertension, hepatitis, adenocarcinoma, breast cancer, coronary heart disease, and hypertension. GSTM5 participates in gluthione metabolism and conjugation as well as metapathway biotransformation and has been known to interact with various genes such as CYP1A1, CYP1B1, CYP2B6, CYP2C19, and ALDH1A3.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBP2-10709

• Glutathione S-transferase Mu 5 Antibodies
• Glutathione S-transferase Mu 5 Lysate
• Glutathione S-transferase Mu 5 Proteins