![Flow Cytometry: GL7 (T and B Cell Activation Marker) Antibody (GL-7) [NBP2-00362] - Analysis using the Biotin conjugate of NBP2-00362. Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.5 ug of Rat IgM Isotype Control Biotin (open histogram) or 0.25ug of Anti-Human/Mouse GL7 (T and B Cell Activation Marker) Biotin (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/GL7-T-and-B-Cell-Activation-Marker-Antibody-GL-7-Flow-Cytometry-NBP2-00362-img0003.jpg "Flow Cytometry: GL7 (T and B Cell Activation Marker) Antibody (GL-7) [NBP2-00362] - Analysis using the Biotin conjugate of NBP2-00362. Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.5 ug of Rat IgM Isotype Control Biotin (open histogram) or 0.25ug of Anti-Human/Mouse GL7 (T and B Cell Activation Marker) Biotin (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | Flow, IHC, IP |
| Clone | GL-7 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | The immunogen for this antibody is T- and B-cell activation antigen (GL7, Ly-77). |
| Isotype | IgM |
| Clonality | Monoclonal |
| Host | Rat |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | The GL-7 (GL7) antibody has been tested by flow cytometric analysis of ConA-activated mouse splenocytes. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The GL-7 antibody has also been reported for use in immunoprecipitation and immunohistochemical staining of frozen tissue sections. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.2) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Flow Cytometry: GL7 (T and B Cell Activation Marker) Antibody (GL-7) [NBP2-00362] - Staining of 3-day unstimulated (left) and 3-day ConA-stimulated (right) BALB/c splenocytes with 0.25 ug of Rat IgM kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Human/Mouse GL7 (T and B Cell Activation Marker) Purified (filled histogram) followed by Anti-Rat IgM PE.](http://images.novusbio.com/fullsize/GL7-T-and-B-Cell-Activation-Marker-Antibody-GL-7-Flow-Cytometry-NBP2-00362-img0001.jpg "Flow Cytometry: GL7 (T and B Cell Activation Marker) Antibody (GL-7) [NBP2-00362] - Staining of 3-day unstimulated (left) and 3-day ConA-stimulated (right) BALB/c splenocytes with 0.25 ug of Rat IgM kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Human/Mouse GL7 (T and B Cell Activation Marker) Purified (filled histogram) followed by Anti-Rat IgM PE.")