![Western Blot: Francisella Tularensis LPS Antibody (FB11) [NB110-7999] - Specificity of mAbs 1A4 and FB11. Inactivated F. tularensis SCHU S4 (lane 1), SCHU S4 deltawbtI (lacks O-antigen, lane 2), LVS (lane 3), F. tularensis subsp. holarctica (lane 4), subsp. novicida (lane 5), F. philomiragia (lane 6), purified B. pseudomallei LPS (lane 7) and E. coli LPS (lane 8) were separated on 12% SDS-PAGE gels and blotted onto nitrocellulose membranes. The membranes then were probed with mAb FB11. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0195308), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Francisella-Tularensis-LPS-Antibody-FB11-Western-Blot-NB110-7999-img0004.jpg "Western Blot: Francisella Tularensis LPS Antibody (FB11) [NB110-7999] - Specificity of mAbs 1A4 and FB11. Inactivated F. tularensis SCHU S4 (lane 1), SCHU S4 deltawbtI (lacks O-antigen, lane 2), LVS (lane 3), F. tularensis subsp. holarctica (lane 4), subsp. novicida (lane 5), F. philomiragia (lane 6), purified B. pseudomallei LPS (lane 7) and E. coli LPS (lane 8) were separated on 12% SDS-PAGE gels and blotted onto nitrocellulose membranes. The membranes then were probed with mAb FB11. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0195308), licensed under a CC-BY license.")
| Immunogen | Hybridoma clone for MAb FB11 has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Fr. tularensis. |
| Epitope | The binding site is located on the O-antigen polysaccharide chain which consists of tetrasaccharide fragments and has the following structure: -4)-D-GalpNAcAN-(1-4)--D-GalpNAcAN-(1-3)--D-QuipNac-(1-2)--Quip4NFm-(1.Tetrasaccharide D-GalpNAcAN-(1 |
| Specificity | Recognizes LPS of virulent and vaccine strains of Fr. tularensis. There is no crossreactivity with Fr. novicida, Br. abortus, Br. suis, Br. melitensis, Br. ovis, Y. pestis, Y. enterocolitica, Y. pseudotuberculosis, E. coli, and V. cholerae. The binding site for MAb FB11 is located on the O-antigen polysaccharide chain which consists of tetrasaccharide fragments and has the following structure: -4)alpha-D-GalpNAcAN-(1-4)-alpha-DGalpNAcAN-( 1-3)-beta-D-QuipNAc-(1-2)-beta-Quip4NFm-(1. Tetrasaccharide D-GalpNAcAN-(1-4)-alpha-D-GalpNAcAN-(1-3)-beta-D-QuipNAc-(1-2)-beta-D-Quip4NFm and trisaccharide D-GalpNAcAN-(1-3)-beta-D-QuipNAc-(1-2)-beta-D-Quip4NFm compete in ELISA for binding MAb FB11 with LPS of Fr. tularensis. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | ELISA: Use at an assay dependent dilution. Can be used in sandwich ELISA. The detection limit for the immunoassay is 0.125 ng/ml.IF: Use at an assay dependent dilution. |
|
| Publications |
|
| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.09% Sodium Azide |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Francisella Tularensis LPS Antibody (NB110-7999)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Francisella Tularensis LPS Antibody (FB11) [NB110-7999] - Western Blot illustrates the specificity of mAb 1A4/ NB110-7999 and FB11 used to probe the membranes. Inactivated F. tularensis SCHU S4 (reference ladder: lane 1), SCHU S4 delta wbtI which lacks O-antigen (lane 2), LVS (lane 3), F. tularensis subsp. holarctica (lane 4), subsp. novicida (lane 5), F. philomiragia (lane 6), purified B. pseudomallei LPS (lane 7) and E. coli LPS (lane 8). All were separated on 12% SDS-PAGE gels were used for separation and were blotted onto nitrocellulose membranes. Panel 3 shows how the Pro-Q emerald 300 LPS staining demonstrates the existence of LPS in these samples.Citation: Nualnoi T, Kirosingh A, Basallo K, Hau D, Gates-Hollingsworth MA, Thorkildson P, et al. (2018) Immunoglobulin G subclass switching impacts sensitivity of an immunoassay targeting Francisella tularensis lipopolysaccharide. PLoS ONE 13(4): e0195308. //doi.org/10.1371/journal.pone.0195308](http://images.novusbio.com/fullsize/Francisella-Tularensis-LPS-Antibody-FB11-Western-Blot-NB110-7999-img0002.jpg "Western Blot: Francisella Tularensis LPS Antibody (FB11) [NB110-7999] - Western Blot illustrates the specificity of mAb 1A4/ NB110-7999 and FB11 used to probe the membranes. Inactivated F. tularensis SCHU S4 (reference ladder: lane 1), SCHU S4 delta wbtI which lacks O-antigen (lane 2), LVS (lane 3), F. tularensis subsp. holarctica (lane 4), subsp. novicida (lane 5), F. philomiragia (lane 6), purified B. pseudomallei LPS (lane 7) and E. coli LPS (lane 8). All were separated on 12% SDS-PAGE gels were used for separation and were blotted onto nitrocellulose membranes. Panel 3 shows how the Pro-Q emerald 300 LPS staining demonstrates the existence of LPS in these samples.Citation: Nualnoi T, Kirosingh A, Basallo K, Hau D, Gates-Hollingsworth MA, Thorkildson P, et al. (2018) Immunoglobulin G subclass switching impacts sensitivity of an immunoassay targeting Francisella tularensis lipopolysaccharide. PLoS ONE 13(4): e0195308. //doi.org/10.1371/journal.pone.0195308")
![Direct ELISA: Francisella Tularensis LPS Antibody (FB11) [NB110-7999] - mAb 1A4/ NB110-7999 antibody was used for a direct ELISA to determine an antibody-antibody interaction. Panel A shows the binding interaction between each mAb subclasses and 1A4 lgG3. Panel B shows the self-association of each mAb 1A4 subclass. Citation: Nualnoi T, Kirosingh A, Basallo K, Hau D, Gates-Hollingsworth MA, Thorkildson P, et al. (2018) Immunoglobulin G subclass switching impacts sensitivity of an immunoassay targeting Francisella tularensis lipopolysaccharide. PLoS ONE 13(4): e0195308. //doi.org/10.1371/journal.pone.0195308](http://images.novusbio.com/fullsize/Francisella-Tularensis-LPS-Antibody-FB11-Direct-ELISA-NB110-7999-img0001.jpg "Direct ELISA: Francisella Tularensis LPS Antibody (FB11) [NB110-7999] - mAb 1A4/ NB110-7999 antibody was used for a direct ELISA to determine an antibody-antibody interaction. Panel A shows the binding interaction between each mAb subclasses and 1A4 lgG3. Panel B shows the self-association of each mAb 1A4 subclass. Citation: Nualnoi T, Kirosingh A, Basallo K, Hau D, Gates-Hollingsworth MA, Thorkildson P, et al. (2018) Immunoglobulin G subclass switching impacts sensitivity of an immunoassay targeting Francisella tularensis lipopolysaccharide. PLoS ONE 13(4): e0195308. //doi.org/10.1371/journal.pone.0195308")
![Sandwich ELISA Capture: Francisella Tularensis LPS Antibody (FB11) [NB110-7999] - Table shows the limit of detection of F. tularensis LPS (ng/mL) for each combination of mAb 1A4 antibody subclass variants determined in antigen capture ELISA. LODs shown are mean +/- standard deviation and the ELISAs were performed in quadruplicate. Bold indicates poorest LOD, derived using mAb 1A4 IgG3 as both capture and detector mAbs. Italics indicate the improved LODs, obtained using non-IgG3 1A4 mAb subclass variants.Citation: Nualnoi T, Kirosingh A, Basallo K, Hau D, Gates-Hollingsworth MA, Thorkildson P, et al. (2018) Immunoglobulin G subclass switching impacts sensitivity of an immunoassay targeting Francisella tularensis lipopolysaccharide. PLoS ONE 13(4): e0195308. //doi.org/10.1371/journal.pone.0195308](http://images.novusbio.com/fullsize/Francisella-Tularensis-LPS-Antibody-FB11-Sandwich-ELISA-Capture-NB110-7999-img0003.jpg "Sandwich ELISA Capture: Francisella Tularensis LPS Antibody (FB11) [NB110-7999] - Table shows the limit of detection of F. tularensis LPS (ng/mL) for each combination of mAb 1A4 antibody subclass variants determined in antigen capture ELISA. LODs shown are mean +/- standard deviation and the ELISAs were performed in quadruplicate. Bold indicates poorest LOD, derived using mAb 1A4 IgG3 as both capture and detector mAbs. Italics indicate the improved LODs, obtained using non-IgG3 1A4 mAb subclass variants.Citation: Nualnoi T, Kirosingh A, Basallo K, Hau D, Gates-Hollingsworth MA, Thorkildson P, et al. (2018) Immunoglobulin G subclass switching impacts sensitivity of an immunoassay targeting Francisella tularensis lipopolysaccharide. PLoS ONE 13(4): e0195308. //doi.org/10.1371/journal.pone.0195308")