FoxP3 Fixation & Permeabilization Flow Cytometry Kit Summary
| Description |
FlowXTM FoxP3 Fixation & Permeabilization Buffer Kit is formulated and optimized for immunofluorescent staining of FoxP3+ single-cell suspensions intended for flow cytometric acquisition and analysis. This product is supplemented with the metabolic inhibitor sodium azide. This product is designed for flow cytometry applications with fluorochrome-conjugated antibodies. Refer to product specification sheets to obtain the recommended working antibody dilutions. |
| Kit Type |
Flow Cytometry Kit |
| Gene |
FOXP3 |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze. |
Kit Components
|
Components
|
- FoxP3 Fixation/ Transcription Factor Diluent
- FoxP3 Fixation/Transcription Factor Concentrate (4X)
- FoxP3/ Transcription Factor
- Permeabilization and Wash Buffer (10X)
|
Background
Human FOXP3 is a 47 kDa member of the P subclass of the forkhead box family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (forkhead) DNA-binding domain. In human, FOXP3 is found in CD4+, CD8+ and CD4+CD25+ T cells and directly associates with NFAT and NFkB, suppressing their activity in CD4+ T cells.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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