Exosome Standards (Human Urine) Summary
| Description |
Highly pure, lyophilized exosome standards with superior stability, optimal for multiple applications including: Assay calibration, Spike-in control for exosome quantification, Protein marker analysis for different techniques such as Western Blot and Flow Cytometry, Extraction and analysis of exosomal RNA and DNA.
Quantity per vial of 30 ug size (number of particles in 30 ug: > 1x10^8). Quantity per vial of 100 ug size (number of particles in 100 ug: > 1x10^10). |
Preparation
Method |
Isolation involves Tangential flow filtration combined with Size Exclusion Chromatogrpahy. Exosomes (small EVs) are quantified and validated for protein content and particle number by Nanoparticle Tracking Analysis as well as for common tetraspanins maker validation. Lyophilization does not alter stability of exosome proteins and nucleic acids. |
Applications/Dilutions
| Dilutions |
- Electron Microscopy
- ELISA
- Flow Cytometry
- Nucleic Acid Extraction
- Western Blot
|
Packaging, Storage & Formulations
| Storage |
Store at 4C. After reconstitution store at -70C. |
| Buffer |
Lyophilized from human urine |
| Concentration |
LYOPH |
| Reconstitution Instructions |
Add deionized water, 100 ul for Standard 100 ug and 30 ul for Standard 30 ug, to get a final concentration of 1 mg/mL. Resuspend exosomes pipetting the solution up and down 10-15 times, avoiding bubbles. Vortex the reconstituted standard for 60 seconds. |
Background
Exosomes are small endosome derived lipid nanoparticles (30-150nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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![Western Blot: Exosome Standards (Human Urine) [NBP2-49840] - Marker detection by Western Blotting. Reconstituted Exosomes can be directly lysed in Laemmli buffer, then loaded on the Electrophoresis gel. Recommended quantity: 10-20 ug per line.](http://images.novusbio.com/fullsize/Exosome-Standards-Human-Urine-Western-Blot-NBP2-49840-img0001.jpg "Western Blot: Exosome Standards (Human Urine) [NBP2-49840] - Marker detection by Western Blotting. Reconstituted Exosomes can be directly lysed in Laemmli buffer, then loaded on the Electrophoresis gel. Recommended quantity: 10-20 ug per line.")
![Nucleic Acid Extraction: Exosome Standards (Human Urine) [NBP2-49840] - Profiling of Exosome associated nucleic acids. Reconstituted Exosomes can be used for profiling nucleic acids (RNAs and DNA) biomarkers. The lysis buffer can be added directly to the Exosome preparation. Recommended quantity: 20-50 ug perreaction.](http://images.novusbio.com/fullsize/Exosome-Standards-Human-Urine-Nucleic-Acid-Extraction-NBP2-49840-img0002.jpg "Nucleic Acid Extraction: Exosome Standards (Human Urine) [NBP2-49840] - Profiling of Exosome associated nucleic acids. Reconstituted Exosomes can be used for profiling nucleic acids (RNAs and DNA) biomarkers. The lysis buffer can be added directly to the Exosome preparation. Recommended quantity: 20-50 ug perreaction.")
