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EV SEC Columns

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Product Details

Summary
Suitable Sample Type
Biofluids or cell culture supernatant

Order Details

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EV SEC Columns Summary

Description

PURE-EVs are size exclusion chromatography (SEC) columns designed to isolate extracellular vesicles (EVs) from small volume samples. Compared to other techniques, PURE-EV efficiently separates EVs from circulating proteins while maintaining original shape and functionality of vesicles. Highly pure EV preparations, no residual chemicals from precipitation, and rapid isolation in approximately 20 minutes. Isolated EVs are suitable for a wide range of downstream analyses such as nanotracking analysis (NTA), protein profiling (western blotting, ELISA, Flow Cytometry), and nucleic acid extraction.

Sample Volume
500 ul - 2 ml

Packaging, Storage & Formulations

Storage
Store at 4C.

Alternate Names for EV SEC Columns

  • EV isolation
  • exosome isolation
  • extracellular vesicle isolation

Background

Size-exclusion chromatography (SEC) separates macromolecules based on their size. To accomplish this, the sample is passed through a porous column and molecules are isolated in fractions from the eluate. The size of the pores can vary depending on the size of the desired product. For isolation of EVs from proteins and lipoproteins, pore sizes can vary from ~40-80 nm. As the sample passes through the column, molecules smaller than the pore size will get trapped in the pores, slowing them down, while larger molecules will pass through. This results in the larger molecules, such as EVs, eluting from the column in earlier fractions, while smaller molecules, such as proteins and protein aggregates, are eluted in later fractions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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