EV Precipitation Solution (Urine) Summary
| Description |
EV Precipitation Solution (Urine) is a simple and rapid method of high yield extracellular vesicle (EV) isolation from urine samples. Based on polymer precipitation technique, samples are incubated with EV Precipitation Solution on ice and exosomes are isolated after a gentle table-top centrifugation step. Advantages of EV Precipitation Solution include: High yield of EVs, No prior sample concentration required, No special equipment. Isolated EVs are suitable for a wide range of downstream analyses such as nanotracking analysis (NTA), protein profiling (western blotting, ELISA, Flow Cytometry), and nucleic acid extraction. |
| Sample Volume |
5-20 mL |
Applications/Dilutions
| Application Notes |
A 30mL solution is equalivant to 25 reactions (urine) |
Packaging, Storage & Formulations
| Storage |
Store at room temperature. |
Alternate Names for EV Precipitation Solution (Urine)
Background
For precipitation techniques to isolate extracellular vesicles, samples containing EVs are incubated with a buffer that changes their solubility or sedimentation rate. One common precipitation buffer uses polyethyleneglycol (PEG), a hydrophilic polymer. By incubating EVs with PEG, it decreases the solubility of EVs in solution and, using a very gentle centrifugation step, causes accumulation of EVs at the bottom of the tube.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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![EV Precipitation Solution (Urine) [NBP3-11766] - Exosomes isolated from 10 ml and 20 ml of urine were resuspended in 24 ul of PBS 1X, and the entire amount was loaded with 6 ul of Laemmli Sample buffer 5X on acrylamide gel. Ultracentrifuged exosomes (30 ug) were used as control. Western blotting performed using anti-Alix (Catalog # NBP3-18364).](http://images.novusbio.com/fullsize/nbp3-11766_ev-precipitation-solution-urine-612025125636..png "EV Precipitation Solution (Urine) [NBP3-11766] - Exosomes isolated from 10 ml and 20 ml of urine were resuspended in 24 ul of PBS 1X, and the entire amount was loaded with 6 ul of Laemmli Sample buffer 5X on acrylamide gel. Ultracentrifuged exosomes (30 ug) were used as control. Western blotting performed using anti-Alix (Catalog # NBP3-18364).")
![EV Precipitation Solution (Urine) [NBP3-11766] - After exosome isolation resuspend pellet in 100 ul of PBS 1X and load the entire amount in a well of an ELISA plate. Example reports CD9 expression in isolated exosomes from 10 ml of urine. 30 ug of purified exosomes via ultracentrifuge (UC) were used as control.](http://images.novusbio.com/fullsize/nbp3-11766_ev-precipitation-solution-urine-612025125812..png "EV Precipitation Solution (Urine) [NBP3-11766] - After exosome isolation resuspend pellet in 100 ul of PBS 1X and load the entire amount in a well of an ELISA plate. Example reports CD9 expression in isolated exosomes from 10 ml of urine. 30 ug of purified exosomes via ultracentrifuge (UC) were used as control.")
