EV MaxiSEC Columns Summary
| Description |
maxiPURE-EVs are size exclusion chromatography (SEC) columns designed to isolate extracellular vesicles (EVs) with the same specificity as PURE-EV, but from larger sample volumes. Advantages of maxiPURE-EVs include: Well suited for large volume of dilute matrices (prior 10x concentration recommended), highly pure EV preparations, no residual chemicals from precipitation, and rapid isolation in approximately 30 minutes. Isolated EVs are suitable for a wide range of downstream analyses such as nanotracking analysis (NTA), protein profiling (western blotting, ELISA, Flow Cytometry), and nucleic acid extraction. |
| Sample Volume |
5 - 20 mL |
Packaging, Storage & Formulations
Alternate Names for EV MaxiSEC Columns
Background
Size-exclusion chromatography (SEC) separates macromolecules based on their size. To accomplish this, the sample is passed through a porous column and molecules are isolated in fractions from the eluate. The size of the pores can vary depending on the size of the desired product. For isolation of EVs from proteins and lipoproteins, pore sizes can vary from ~40-80 nm. As the sample passes through the column, molecules smaller than the pore size will get trapped in the pores, slowing them down, while larger molecules will pass through. This results in the larger molecules, such as EVs, eluting from the column in earlier fractions, while smaller molecules, such as proteins and protein aggregates, are eluted in later fractions.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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