Bovine ESTRIOL ELISA Kit (Colorimetric) Summary
| Specificity |
This kit recognizes Bovine Estriol in samples. No significant cross-reactivity or interference between Bovine Estriol and analogues was observed. |
| Standard Curve Range |
3.13-200 pg/mL |
| Sensitivity |
1.11 pg/mL |
| Assay Type |
Competitive-ELISA |
| Inter-Assay |
%CV < 5.54 |
| Intra-Assay |
%CV < 5.76 |
| Spike Recovery |
87-104% |
| Sample Volume |
50 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Concentrated HRP Linked Detection Ab (100x)
- Concentrated Wash Buffer (25x)
- HRP Linked Ab Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
|
Alternate Names for Bovine ESTRIOL ELISA Kit (Colorimetric)
Background
Estriol is a hydroxylated metabolite of ESTRADIOL or ESTRONE that has a hydroxyl group at C3-beta, 16-alpha, and 17-beta position. Estriol is the main estrogen in the blood and urine of pregnant females. During pregnancy, large amount of estriol is produced by the placenta. Serum estriol determinations reflect the status of the fetus and are used to evaluate fetal distress and placental function. Estriol increases until term, reaches a plateau at the 40th week of gestation and then declines. Isomers with inversion of the hydroxyl group or groups are called epiestriol. It is used in the treatment of osteoporosis in combination with Vitamin D. Its expression also seems to be one of the direct causes of preterm labour, together with cervical ultrasound mesurements and espression of Foetal Fibronectin.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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Blogs on ESTRIOL
![Bovine Estriol ELISA Kit (Colorimetric) [NBP3-23542] - Samples were spiked with high concentrations of Bovine Estriol and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-23542_bovine-estriol-elisa-kit-colorimetric-1010202316534921.jpg "Bovine Estriol ELISA Kit (Colorimetric) [NBP3-23542] - Samples were spiked with high concentrations of Bovine Estriol and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Bovine ESTRIOL ELISA Kit (Colorimetric) [NBP3-23542] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-23542_bovine-estriol-elisa-kit-colorimetric-4320248595142.jpg "ELISA: Bovine ESTRIOL ELISA Kit (Colorimetric) [NBP3-23542] - Standard Curve Reference")
