EL4 Whole Cell Lysate Summary
| Description |
GROWTH MEDIA: DMEM & 10% horse serum (equine serum) |
| Specificity |
EL4 (T lymphocyte; Lymphoma) |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles. |
| Buffer |
SDS sample buffer containing 50 mM DTT. |
| Concentration |
2 mg/ml |
Lysate Details for Array
| Type |
Cell |
| Tissue |
Blood Lymph |
| Tissue Condition |
Lymphoma |
| Subcellular Fraction |
Whole |
Background
EL4 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The EL4 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
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