![Western Blot: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis of DYKDDDDK tagged protein demonstrating that the rat monoclonal DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] is 10-15 fold more sensitive than Sigma's M2 mouse ANTI-FLAG M2 antibody.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-L5-Western-Blot-NBP1-06712-img0010.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis of DYKDDDDK tagged protein demonstrating that the rat monoclonal DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] is 10-15 fold more sensitive than Sigma's M2 mouse ANTI-FLAG M2 antibody.")
| Reactivity | ETSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | L5 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Immunogen | DYKDDDDK Epitope Tag Antibody (L5) was made to N-terminal DYKDDDDK-tagged extracellular domain of mouse Langerin. Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody. |
| Epitope | DDDDK |
| Specificity | Binds to same epitope as Sigma's Anti-FLAGM2 Antibody. FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Rat |
| Purity | Tissue culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 1.01 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tissue culture supernatant |
| Preservative | 0.1% Sodium Azide |
| Purity | Tissue culture supernatant |
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reviewed by:
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WB | Human | 01/28/2022 |
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WB | Human | 04/04/2020 |
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WB | Human | 12/27/2019 |
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WB | Human | 02/18/2019 |
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IP | Human | 03/30/2017 |
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IF | Rat | 06/02/2015 |
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WB | Human | 03/13/2015 |
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reviewed by:
Matt Stratton |
WB | Other | 06/11/2014 |
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IF | Human | 05/27/2010 |
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reviewed by:
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WB | Other | 03/31/2010 |
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Secondary Antibodies |
Isotype Controls |
Research Areas for DYKDDDDK Epitope Tag Antibody (NBP1-06712)Find related products by research area.
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Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto... Read full blog post. |
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Hoist the DYKDDDDK Epitope Tag The DYKDDDDK Epitope Tag antibody was raised against a short, artificial sequence that is widely and commonly used as a protein fusion tag. It is pervasively present in a vast amount of scientific studies, and is a very powerful investigative research... Read full blog post. |
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| Verified Customer 01/28/2022 |
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| Application: | WB | |
| Species: | Human |
| Verified Customer 04/04/2020 |
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| Application: | WB | |
| Species: | Human |
| Verified Customer 12/27/2019 |
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| Application: | WB | |
| Species: | Human |
![Western Blot: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis of DYKDDDDK tagged SFO-p41 protein with DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712]. Observed molecular weight ~46.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-L5-Western-Blot-NBP1-06712-img0005.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis of DYKDDDDK tagged SFO-p41 protein with DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712]. Observed molecular weight ~46.")
![Western Blot: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis of DYKDDDDK tagged AIFm2 protein (A), DYKDDDDK tagged IREB2 protein (B), and vector control (C) using DYKDDDDK epitope tag antibody (L5) [NBP1-06712] at 2 ug/ml.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-L5-Western-Blot-NBP1-06712-img0007.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis of DYKDDDDK tagged AIFm2 protein (A), DYKDDDDK tagged IREB2 protein (B), and vector control (C) using DYKDDDDK epitope tag antibody (L5) [NBP1-06712] at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis of HeLa cells transfected with a FLAG encoded construct (top) or untransfected (bottom), using DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712]. Image from verified customer review.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-L5-Immunocytochemistry-Immunofluorescence-NBP1-06712-img0009.jpg "Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis of HeLa cells transfected with a FLAG encoded construct (top) or untransfected (bottom), using DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712]. Image from verified customer review.")
![Immunohistochemistry: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Third instar larva of the genotype [hs-Flp; nrv2-Gal4/MCFO-2]. flp expression of the multicolor FlpOut construct was induced by 1 h 37C heat shock during first instar stage. Larvae were stained for HA (green), V5 (red), and FLAG (blue). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32901033/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-L5-Immunohistochemistry-NBP1-06712-img0011.jpg "Immunohistochemistry: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Third instar larva of the genotype [hs-Flp; nrv2-Gal4/MCFO-2]. flp expression of the multicolor FlpOut construct was induced by 1 h 37C heat shock during first instar stage. Larvae were stained for HA (green), V5 (red), and FLAG (blue). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32901033/) licensed under a CC-BY license.")
![Western Blot: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Detection of DDK in SUSD3 overexpression lysate using [NBP1-06712] followed by goat anti-rat IgG HRP conjugated secondary antibody (cat.# NB7115). Image from verified customer review.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-L5-Western-Blot-NBP1-06712-img0008.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Detection of DDK in SUSD3 overexpression lysate using [NBP1-06712] followed by goat anti-rat IgG HRP conjugated secondary antibody (cat.# NB7115). Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: Rat Monoclonal DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis using DyLight 488 conjugated antibody (NBP1-06712G). Normal mouse prostate cells overexpressed with Flag-tagged construct (Green: Flag). Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-06712_rat-monoclonal-dykddddk-epitope-tag-antibody-l5-immunocytochemistry-immunofluorescence-792023163813..jpg "Immunocytochemistry/Immunofluorescence: Rat Monoclonal DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Analysis using DyLight 488 conjugated antibody (NBP1-06712G). Normal mouse prostate cells overexpressed with Flag-tagged construct (Green: Flag). Image from verified customer review.")
![Immunohistochemistry: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Generation of a wrapping glia driver.Confocal projection of larval filet preparations of the genotypes indicated. Representative images are shown taken from >10 animals analyzed for each genotype. a Third instar larva with the genotype [nrv2-stGFP]. Broad GFP expression is detected in the CNS. Note the restricted expression in the peripheral nervous system which corresponds to the wrapping glia (arrowheads). b Third instar larva of the genotype [hs-Flp; nrv2-Gal4/MCFO-2]. flp expression of the multicolor FlpOut construct was induced by 1 h 37 °C heat shock during first instar stage. Larvae were stained for HA (green), V5 (red), & FLAG (blue). c Same animal as in (a). Expression of stRed is observed only in the CNS & no expression is found in the wrapping glia. d–f Overlay of nrv2-GFP (green) & 90C03 > dsRed (red) expression. Note the complete overlap of dsRed (e) & GFP expression in the CNS (f). g Young third instar larva with the genotype [hs-Flp; nrv2-Gal4/MCFO-2; 90C03-Gal80]. flp expression was induced by 1 h 37 °C heat shock during first instar stage. Larvae were stained for HA (green), V5 (red), & FLAG (blue). h Living third instar larva of the genotype [nrv2-Gal4, UAS-CD8GFP; 90C03-Gal80/90C03-Gal80]. Note strong expression at the anterior tip of the larva. Scale bars are 250 µm (a–c, g, h) & 100 µm (d–f). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32901033), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06712_rat-monoclonal-dykddddk-epitope-tag-antibody-l5-310202416182637.jpg "Immunohistochemistry: DYKDDDDK Epitope Tag Antibody (L5) [NBP1-06712] - Generation of a wrapping glia driver.Confocal projection of larval filet preparations of the genotypes indicated. Representative images are shown taken from >10 animals analyzed for each genotype. a Third instar larva with the genotype [nrv2-stGFP]. Broad GFP expression is detected in the CNS. Note the restricted expression in the peripheral nervous system which corresponds to the wrapping glia (arrowheads). b Third instar larva of the genotype [hs-Flp; nrv2-Gal4/MCFO-2]. flp expression of the multicolor FlpOut construct was induced by 1 h 37 °C heat shock during first instar stage. Larvae were stained for HA (green), V5 (red), & FLAG (blue). c Same animal as in (a). Expression of stRed is observed only in the CNS & no expression is found in the wrapping glia. d–f Overlay of nrv2-GFP (green) & 90C03 > dsRed (red) expression. Note the complete overlap of dsRed (e) & GFP expression in the CNS (f). g Young third instar larva with the genotype [hs-Flp; nrv2-Gal4/MCFO-2; 90C03-Gal80]. flp expression was induced by 1 h 37 °C heat shock during first instar stage. Larvae were stained for HA (green), V5 (red), & FLAG (blue). h Living third instar larva of the genotype [nrv2-Gal4, UAS-CD8GFP; 90C03-Gal80/90C03-Gal80]. Note strong expression at the anterior tip of the larva. Scale bars are 250 µm (a–c, g, h) & 100 µm (d–f). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32901033), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
