Applications/Dilutions
| Dilutions |
- ELISA 1-2 ug/ml
- Sandwich ELISA 1-2 ug/ml
|
| Application Notes |
ELISA data: Capture Antibody ChABC/6A12 [NBP1-96142] was bound to a 96-well ELISA plate and serial dilutions of ChABC were added in physiological buffer. Bound ChABC was labeled using Detection Antibody biotinylated ChABC/1E10 [NBP1-96141B]. Bound Detection Antibody was labeled with avidin-HRP and the assay developed with chromagen OPDA/ H2O2. |
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze. |
| Buffer |
NBP1-96141B: PBS with No Preservative
NBP1-96142: Tris-glycine, 150mM NaCl pH 7.5 and 0.05% Sodium Azide |
Kit Components
Alternate Names for ChABC Antibody Pair
Background
ChABC (Chondroitinase ABC) is a bacterial enzyme that has been used for decades, mostly for biochemical characterization of chondroitin sulfate proteoglycans (CSPG). This enzyme degrades the sugar chains attached to the proteoglycan core protein. More recently, ChABC has been used to therapeutically degrade and inactivate CSPG in vivo. CSPGs are involved in a variety of biological processes and play a major inhibitory role in regeneration in the nervous system. For instance, CSPGs have been implicated in preventing regeneration after spinal cord injury. Injection of ChABC at the site of spinal cord injury has improved recovery in numerous animal models. ChABC antibodies serve as tools to help determine the ChABC distribution and content in tissues.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are
guaranteed for 6 months from date of receipt.
Publications for ChABC Antibody Pair (NBP1-97919) (0)
There are no publications for ChABC Antibody Pair (NBP1-97919).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for ChABC Antibody Pair (NBP1-97919) (0)
There are no reviews for ChABC Antibody Pair (NBP1-97919).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
View specific protocols for ChABC Antibody Pair (NBP1-97919):
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for ChABC Antibody Pair (NBP1-97919) (0)
Additional ChABC Products
Research Areas for ChABC Antibody Pair (NBP1-97919)
Find related products by research area.
|
Blogs on ChABC
![ELISA: ChABC Antibody Pair [NBP1-97919] - Standard curve for serial dilutions of ChABC. See Notes for additional information.](http://images.novusbio.com/fullsize/ChABC-Antibody-Pair-ELISA-NBP1-97919-img0001.jpg "ELISA: ChABC Antibody Pair [NBP1-97919] - Standard curve for serial dilutions of ChABC. See Notes for additional information.")
