CD63 Immunobeads for Exosome Isolation Summary
| Description |
CD63 Immunocapture Beads are 0.4 um latex beads covalently coupled with antibodies against extracellular vesicle (EV) surface antigen CD63. Samples containing EVs are incubated with Immunocapture Beads for rapid and specific isolation of EVs expressing CD63.
Using the supplied Elution Buffer, EVs can be eluted from Immunocapture beads and used in downstream analyses such as protein profiling and nucleic acid extraction.
Immunobeads can be re-used after incubation with Bead Regeneration Buffer. |
| Sample Volume |
0.5-1 mL |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
|
Components
|
- CD63 Immunocapture BeadsExosome Elution BufferBead Regeneration Buffer
|
Alternate Names for CD63 Immunobeads for Exosome Isolation
Background
Immunocapture for isolation of exosomes relies on the antigen-antibody interaction. Antibodies directed at target of interest (eg CD9, CD63) are coated either in a 96-well plate or conjugated to a bead. The sample is applied directly into coated well, or into sample with coated beads, and EVs are allowed to bind. In a final step, EVs can be eluted from the antibody and used in downstream functional and phenotypic analysis. This method of isolation is highly specific, resulting in extremely pure EV populations.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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![CD63 Immunobeads for Exosome Isolation [NBP3-11769] - Alix expression by western blotting of exosomes captured on NBP3-11769 immunobeads from COLO1 cell supernatant vs isotype coupled beads.](http://images.novusbio.com/fullsize/nbp3-11769_cd63-immunobeads-for-exosome-isolation-17122024156300.png "CD63 Immunobeads for Exosome Isolation [NBP3-11769] - Alix expression by western blotting of exosomes captured on NBP3-11769 immunobeads from COLO1 cell supernatant vs isotype coupled beads.")
