Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric) Summary
| Specificity |
This kit recognizes Human CA3 in samples. No significant cross-reactivity or interference between Human CA3 and analogues was observed. |
| Standard Curve Range |
3.13 - 200 ng/mL |
| Sensitivity |
1.88 ng/mL |
| Assay Type |
Sandwich-ELISA |
| Inter-Assay |
CV% < 6.03% |
| Intra-Assay |
CV% < 5.97% |
| Spike Recovery |
87-106% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
CA3 |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
|
Alternate Names for Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric)
Background
Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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Blogs on Carbonic Anhydrase III/CA3
![ELISA: Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric) [NBP2-70057] - Samples were spiked with high concentrations of Human Carbonic Anhydrase III/CA3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-70057_human-carbonic-anhydrase-iii-ca3-elisa-kit-colorimetric-13220241623237.jpg "ELISA: Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric) [NBP2-70057] - Samples were spiked with high concentrations of Human Carbonic Anhydrase III/CA3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric) [NBP2-70057] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-70057_human-carbonic-anhydrase-iii-ca3-elisa-kit-colorimetric-4320249141639.jpg "ELISA: Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric) [NBP2-70057] - Standard Curve Reference")