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Bromodeoxyuridine/BrdU Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [NB500-235] - IF analysis of BrdU in Proteinase K pretreated mouse lacrimal gland. Image courtesy of anonymous customer review.
Immunoprecipitation: Bromodeoxyuridine/BrdU Antibody [NB500-235] - Lysate was immunoprecipitated with anti-brdu antibody (NB500-235), 5-methyl cytosine (5-Me), or a no antibody control (No A). An ELISA was then ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, All, GpSpecies Glossary
Applications Func, ICC/IF, IHC, IP, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
3.2 mg/ml

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Bromodeoxyuridine/BrdU Antibody Summary

Immunogen
Bromodeoxyuridine (BrdU) coupled to keyhole limpet hemocyanin (KLH).
Marker
Proliferation Marker
Specificity
BrdU
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 25-100 ug/ml
  • In vitro assay
Application Notes
Use in IHC-Fr reported in scientific literature (PMID:33731750). Use in IHC-FrFl reported in scientific literature (PMID:33744708) In vitro reported in (PMID: 29100301).
Reviewed Applications
Read 2 Reviews rated 5
using
NB500-235 in the following applications:

Publications
Read Publications using
NB500-235 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33731750).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Concentration
3.2 mg/ml
Purity
Protein G purified

Alternate Names for Bromodeoxyuridine/BrdU Antibody

  • 5-bromo-2-deoxyuridine
  • BrdU
  • Bromodeoxyuridine
  • BUdr

Background

BrdU (Bromodeoxyuridine) is a synthetic nucleoside that is incorporated into actively replicating cells' DNA. In many studies, BrdU incorporation is used as a marker for the proliferation of cells. This technique is particularly valuable in detecting proliferation in tissues that are not easily accessible to other assay types, like the brain. It is also commonly used to detect developmental progress in embryogenesis. Detection of the concentration and pattern of BrdU incorporation in tissues can give clues as to the pattern, rate, and progression of cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bromodeoxyuridine/BrdU Antibody (NB500-235)(24)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Guinea Pig, Rabbit.

We have publications tested in 6 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-FrFl, IHC-P, In vitro.


Filter By Application
ICC/IF
(5)
IF/IHC
(6)
IHC-Fr
(2)
IHC-FrFl
(1)
IHC-P
(1)
In vitro
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(4)
Rat
(2)
Guinea Pig
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NB500-235 Applications Species
Li J, Wang X, Shi L et al. A Mammalian Conserved Circular RNA CircLARP1B Regulates Hepatocellular Carcinoma Metastasis and Lipid Metabolism Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023-11-12 [PMID: 37953462]
Ralph J S van Mechelen, Phani Sudarsanam, Christian J F Bertens, Mehmet O Tas, Marion J J Gijbels, Leonard Pinchuk, Jan de Boer, Henny J M Beckers The Influence of Design Modifications and Microstructured Surface Topographies on Bleb Survival after Glaucoma Tube Shunt Implantation. Advanced biology 2023-04-27 [PMID: 37102630]
van Mechelen RJS, Wolters JEJ, Herfs M et al. Wound Healing Response After Bleb-Forming Glaucoma Surgery With a SIBS Microshunt in Rabbits Translational vision science & technology 2022-08-01 [PMID: 36018582] (IHC-P, Rabbit) IHC-P Rabbit
Luo O, Kwiecien-Delaney B, Martin P et al. The effect of early life immune challenge on adult forced swim test performance and hippocampal neurogenesis Journal of Neuroimmunology 2021-02-01 [PMID: 33744708] (IHC-FrFl, Mouse) IHC-FrFl Mouse
Zachar G, Kemecsei R, Papp SM et al. D-Aspartate consumption selectively promotes intermediate-term spatial memory and the expression of hippocampal NMDA receptor subunits Scientific reports 2021-03-17 [PMID: 33731750] (IHC-Fr, Mouse) IHC-Fr Mouse
Chen ZC, Wang TT, Bian W et al. Allopregnanolone restores the tyrosine hydroxylase-positive neurons and motor performance in a 6-OHDA-injected mouse model CNS Neurosci Ther 2020-06-30 [PMID: 32602622] (IF/IHC, Mouse) IF/IHC Mouse
Redecker TM, Kisko TM, WOhr M, Schwarting RKW Cacna1c haploinsufficiency lacks effects on adult hippocampal neurogenesis and volumetric properties of prefrontal cortex and hippocampus in female rats Physiol. Behav. 2020-05-27 [PMID: 32473156] (IF/IHC, Rat) IF/IHC Rat
Karakashev S, Fukumoto T, Zhao B et al. EZH2 Inhibition Sensitizes CARM1-High, Homologous Recombination Proficient Ovarian Cancers to PARP Inhibition Cancer Cell 2020-01-22 [PMID: 32004442] (ICC/IF, Human) ICC/IF Human
Nicole Lawrence, Adelaide S M Dennis, Adele M Lehane, Anna Ehmann, Peta J Harvey, Aurélie H Benfield, Olivier Cheneval, Sónia Troeira Henriques, David J Craik, Brendan J McMorran Defense Peptides Engineered from Human Platelet Factor 4 Kill Plasmodium by Selective Membrane Disruption. Cell chemical biology 2019-06-12 [PMID: 30033131]
Xu H, Zhang L, Chen W et al. Inhibitory effect of caveolin-1 in vascular endothelial cells, pericytes and smooth muscle cells Oncotarget. 2017-09-29 [PMID: 29100301] (In vitro, Human) In vitro Human
Show All 24 Publications.

Reviews for Bromodeoxyuridine/BrdU Antibody (NB500-235) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB500-235:
Filter by Applications
IF
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Bromodeoxyuridine/BrdU NB500-235
Enlarge
5
reviewed by:
Claire Kublin
IF Mouse 03/15/2011
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse Lacrimal Gland
SpeciesMouse
  5
reviewed by:
Verified Customer
IHC-Fr Mouse 05/27/2009
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmouse brain tissue section (dorsal striatum)
SpeciesMouse
LotP13083008

Product General Protocols

View specific protocols for Bromodeoxyuridine/BrdU Antibody (NB500-235): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Bromodeoxyuridine/BrdU Antibody (NB500-235). (Showing 1 - 1 of 1 FAQs).

  1. I have been using your BrdU antibody for some time now and saw that you have discontinued it. I was wondering if you had any alternative antibodies that would also work? it is antibody NB500-169 lot# 1706-5
    • We recommend this product: NB500-235​

Secondary Antibodies

 

Isotype Controls

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Research Areas for Bromodeoxyuridine/BrdU Antibody (NB500-235)

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Blogs on Bromodeoxyuridine/BrdU.

Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein
By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto...  Read full blog post.

BrDU (Bromodeoxyuridine)
The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of repeated use in molecular and cytokinetic studies, as detailed in reviews by Vanderlaan and Dolbeare (1,2).  Because BrDU is only incorporated in...  Read full blog post.

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Recent Reviews

5
5
2
4
0
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2
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1
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Claire Kublin
03/15/2011
Application: IF
Species: Mouse

 
Verified Customer
05/27/2009
Application: IHC-Fr
Species: Mouse