![Human Breast Tissue Lysate (Adult Tumor) [NBP2-28004]](http://images.novusbio.com/fullsize/Human-Breast-Tissue-Lysate-Adult-Tumor-NBP2-28004-img0001.jpg "Human Breast Tissue Lysate (Adult Tumor) [NBP2-28004]")
| Reactivity | HuSpecies Glossary |
| Concentration | 1 mg/ml |
| Description | The vial is provided with a 10% overfill. Maximum recovery can be obtained by centrifuging the vial briefly to collect any solution on the cap and tube sides. |
| Specificity | Sex:female Age :55 Diagnosis:Solid adenocarcinoma Grade:3 Stage:II TNM:T2N1M0 Clinical Tissue Human Breast Tumor Tissue Lysate, Soluble protein fraction |
| Preparation Method |
Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature. |
| Application Notes | These lysates are proteomic discovery tools.Researchers should validate and optimize for individual use. Potential applications MAY include WB, immunoprecipitation, protein-protein interactions, ligand binding, ELISA. Note: For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required. |
| Storage | Store at -20C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles. |
| Concentration | 1 mg/ml |
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![Human Breast Tissue Lysate (Adult Tumor) [NBP2-28004]](http://images.novusbio.com/fullsize/Human-Breast-Tissue-Lysate-Adult-Tumor-NBP2-28004-img0002.jpg "Human Breast Tissue Lysate (Adult Tumor) [NBP2-28004]")