Human BNP ELISA Kit (Colorimetric) Summary
| Specificity |
This kit recognizes Human BNP in samples. No significant cross-reactivity or interference between Human BNP and analogues was observed. |
| Standard Curve Range |
31.25 - 2000 pg/mL |
| Sensitivity |
18.75 pg/mL |
| Assay Type |
Competitive-ELISA |
| Inter-Assay |
CV% < 4.66% |
| Intra-Assay |
CV% < 5.37% |
| Spike Recovery |
90-106% |
| Sample Volume |
50 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
NPPB |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
|
Alternate Names for Human BNP ELISA Kit (Colorimetric)
Background
Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C-type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides, see fig. 18). BNP is a 32 amino acid peptide with disulfide bond between the cystein residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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Blogs on BNP
![ELISA: Human BNP ELISA Kit (Colorimetric) [NBP2-70010] - Samples were spiked with high concentrations of Human BNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-70010_human-bnp-elisa-kit-colorimetric-13220241620464.jpg "ELISA: Human BNP ELISA Kit (Colorimetric) [NBP2-70010] - Samples were spiked with high concentrations of Human BNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human BNP ELISA Kit (Colorimetric) [NBP2-70010] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-70010_human-bnp-elisa-kit-colorimetric-432024963961.jpg "ELISA: Human BNP ELISA Kit (Colorimetric) [NBP2-70010] - Standard Curve Reference")