Amine Reactive Comp-Bead 2 Population Kit Summary
| Description |
The Amine Reactive Compensation Beads are designed for labeling amine-reactive dyes (live/dead fixable viability stains) when setting compensation in flow cytometry.
- Ensure consistent and accurate compensation in any channel
- Evaluate cell viability of animal cells, bacteria, yeast, and fungi
- Contains both amine reactive and nonreactive beads for generating positive and negative populations
Concentration: 10^7 particles / mL
Particle size: 7.0 -7.9 micron
Particle material: polystyrene |
| Kit Type |
Kit |
Applications/Dilutions
| Dilutions |
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| Application Notes |
Dye and bead concentrations may be further optimized for best results. Centrifugation force and time may need to be increased if needed. Protect the beads from light after exposure to the dye. Use immediately after staining. |
| Reviewed Applications |
Read 1 Review rated 4 using
NBP3-00496 in the following application:
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Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. Do not freeze. |
| Buffer |
0.016 M PBS (pH 7.4) |
| Preservative |
0.02% Sodium Azide |
Kit Components
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Components
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- High Binding Beads - 5 mL
- Negative Binding Beads - 5 mL
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Alternate Names for Amine Reactive Comp-Bead 2 Population Kit
Background
Compensation is the mathematical correction for the amount of overlap (spillover) of one fluorochrome's emission spectra into another fluorochrome's channel. To calculate this overlap, record single-color stained controls for each fluorochrome in a multicolor panel. This allows the cytometer to visualize the emission of each fluorochrome in each channel, or detector, and subtract the spillover, generating a matrix to apply to multi-color experimental samples. To ensure accurate calculations, single-color controls need to be as bright, or brighter, than the marker on the cell of interest. With increased use of multi-parametric flow cytometry and the large number of fluorochromes available, it is more important than ever to ensure accurate compensation for reliable interpretation of data and reproducible experimental results.
Compensation beads are a highly desirable alternative to traditional cell-based single-color compensation. Positive beads can come pre-loaded with anti-IgG for conjugated antibody capture of mouse, rat, or hamster origin. Uncoated (negative) beads ensure standardization of the autofluorescence in each channel. Beads are preferable to cell-based compensation for several reasons:
1) No precious sample is wasted, and more sample can be used for experimental acquisition.
2) Since 100% of positive beads are able to capture the antibody, the exact experimental fluorochrome can be used regardless of a marker's cellular expression. Dimly expressed markers may not be bright enough for compensation using cells.
3) Reduced autofluorescence of beads allows for more precise calculation of spectral overlap.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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Review for Amine Reactive Comp-Bead 2 Population Kit (NBP3-00496) (1)
41
Average Rating: 4 (Based on 1 review)
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| Images |
Ratings |
Applications |
Species |
Date |
Details |
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4
reviewed by:
Shinford Sun
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09/16/2024 |
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Product General Protocols
View specific protocols for Amine Reactive Comp-Bead 2 Population Kit (NBP3-00496):
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Blogs on Amine Reactive Comp-Bead 2 Population
![Amine Reactive Comp-Bead 2 Population [NBP3-00496] - Histogram. Amine Reactive Comp-Beads stained with Biolegend Zombie Green(Tm).](http://images.novusbio.com/fullsize/Amine-Reactive-Comp-Bead-2-Population-NBP3-00496-img0001.jpg "Amine Reactive Comp-Bead 2 Population [NBP3-00496] - Histogram. Amine Reactive Comp-Beads stained with Biolegend Zombie Green(Tm).")
![Flow Cytometry: Amine Reactive Comp-Bead 2 Population Kit [NBP3-00496] - Beads used for flow cytometry compensation calculations. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp3-00496_amine-reactive-comp-bead-2-population-kit-flow-cytometry-1892024144937..png "Flow Cytometry: Amine Reactive Comp-Bead 2 Population Kit [NBP3-00496] - Beads used for flow cytometry compensation calculations. Image from a verified customer review.")
