AbSelect™ Antibody Concentration and Clean Up Antibody Purification Kit Summary
Description
Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions with enzymes, biotin, streptavidin and fluorophores.
Features
Benefits
Quickly and easily concentrate antibodies and proteins
Easy and quick to use
Remove non-protein additives such as sodium azide, tris and glycine
High recoveries, around 60 - 100%
Perform a simple buffer exchange for more favourable conjugation conditions
Suitable for Lightning-Link kits and for other kits
The antibody concentration and clean-up kit method utilizes a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution.
Kit Type
Antibody Purification Kit
Applications/Dilutions
Reviewed Applications
Read 1 Review rated 4 using 861-0010 in the following application:
This product is manufactured by Abcam and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.
Background
The Antibody concentration and Clean Up Kit allows for the quick and easy concentration of antibodies and proteins. Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions with enzymes, biotin, streptavidin and fluorophores. The kit can also be used to reduce the concentration of many unwanted additives often found in antibody formulations such as azide, glycine or tris. The antibody clean up kit method utilizes a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution. The clean up kit also allows the experimenter to perform a simple buffer exchange to transfer the antibody into a more favorable conjugation buffer.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
Publications for AbSelect (TM) Antibody Concentration and Clean Up Kit (861-0010) (0)
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Review for AbSelect (TM) Antibody Concentration and Clean Up Kit (861-0010) (1)
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FAQs for AbSelect (TM) Antibody Concentration and Clean Up Kit (861-0010). (Showing 1 - 1 of 1 FAQ).
Is the NB500-169 still available in the concentration of 2mg/ml? We ordered five vials in the past month with the unit size 0.1 mg. Three of them arrived with a concentration of 2mg/ml, the last two vial with a concentration of 1mg/ml. Can we influence the concentration when ordering?
In terms of the concentration of the vials, unfortunately sometimes the concentrations of our products may change. (The concentration of each vial is that stated on the label.) I am sorry, but it is not possible for you to order specifically 2 mg/ml vials of NB500-169. (If you require a concentration of 2 mg/ml, you may be interested in our Antibody Concentration and Clean Up Kit (product code for 1 spin column is 861-0005; 3 spin columns is 861-0010), which you could use to reduce the volume of the antibody by half and hence double the concentration.)
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