![Flow Cytometry: 7-Aminoactinomycin D Solution [NBP2-29446] - Mouse lymphocytes were unstained (left) or stained (right) with 7-AAD. Without 7-AAD, dead cells cannot be visualized. With 7-AAD, dead cells can be visualized and excluded from further analysis.](http://images.novusbio.com/fullsize/7-Aminoactinomycin-D-Solution-Flow-Cytometry-NBP2-29446-img0005.jpg "Flow Cytometry: 7-Aminoactinomycin D Solution [NBP2-29446] - Mouse lymphocytes were unstained (left) or stained (right) with 7-AAD. Without 7-AAD, dead cells cannot be visualized. With 7-AAD, dead cells can be visualized and excluded from further analysis.")
| Applications | Flow |
| Description | Appearance (Form) Powder Appearance (Color) Red to Dark Purple Solubility (Solvent) Chloroform Solubility (Conc) 0.95 - 1.05 mg/ml Solubility (Turbidity) Clear Solubility (Color) Red Purity (HPLC) > 95.50% |
| Specificity | 7-Aminoactinomycin D Solution Molecular Weight 1270.43 |
| Dilutions |
|
|
| Application Notes | Fluorescent DNA stain 7- Amino-Actinomycin D (7-AAD) can be used as a viability probe for methods of nonviable cell exclusion based on light scatter and uptake of the reagent as detected in FL3. 7-AAD is excluded by viable cells but can penetrate cell membranes of dying or dead cells, in which it intercalates into double stranded nucleic acids. It can be used in place of Propidium Iodide (PI) for omission of nonviable cells in flow cytometric assay. The advantage of 7-AAD over PI is that detection is in the far red range of the spectrum (650 nm long-pass filter) and is read in the FL3, compared to PI detection in FL2. This allows this solution to be used in combination with PE (phycoerythrin), FITC (fluorescein isothiocyanate), and APC (allophycocyanin) conjugated antibodies, as well as any other conjugated antibody that does not emit into FL3. |
|
| Publications |
|
| Storage | Store at 4C in the dark. |
| Buffer | PBS and 1.0% FBS |
| Preservative | 0.09% Sodium Azide |
![7-Aminoactinomycin D Solution [NBP2-29446] - 7-Aminoactinomycin D [NBP2-29446]](http://images.novusbio.com/fullsize/7-Aminoactinomycin-D-Solution-NBP2-29446-img0003.jpg "7-Aminoactinomycin D Solution [NBP2-29446] - 7-Aminoactinomycin D [NBP2-29446]")