| Applications | IHC |
| Dilutions |
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| Application Notes | The antigen retrieval protocol is recommended for use in tissues that have been fixed in formalin only. Ensure that the fixed sections are adequately embedded in paraffin. Cut tissue sections to 4-5 microns. Preparation of Working Solutions 1. The 10X concentrated format should be diluted tenfold with distilled or deionized water. 2. Mix one part of concentrated Antigen Retrieval Solution with nineparts of deionized or distilled water. 3. Shake the bottle vigorously to completely mix the components of theconcentrate (the solution may separate into phases over time). 4. Store with cap tightly secured. Protocol Recommendations 1. Deparaffinize and rehydrate tissue sections. 2. Place slides into 1X retrieval solution in a slide container (e.g. Coplinjar, Tissue-Tek, staining dish or metal slide canister). 3. Retrieve sections under pressure 4. After take-off reagent jar containing slides from pressure cooker, allow the slides to cool for 20 minutes to reach room temperature. 5. Wash slides in deionized water and then with wash buffer. Proceed with immunostaining recommendations in the antibody datasheet. 6. Gently rinse by gradually adding DI water to the solution, thenremove slides and rinse with DI water. |
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| Publications |
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| Storage | Store at room temperature. |
| Buffer | Dilute one part buffer with nine parts de-ionized or distilled water. |
| Preservative | No Preservative |
| Publication using NB900-66730 | Applications | Species |
|---|---|---|
| Dhahri H, Matveeva E, Fondufe-Mittendorf Y Approach to Measuring the Effect of PARP1 on RNA Polymerase II Elongation Rates Methods in molecular biology (Clifton, N.J.) 2022-12-14 [PMID: 36515843] |
