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Golgi Localization Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Golgi Localization Pathway and Malignant Neoplasms, Neoplasms, Chimera Disorder, Tissue Adhesions, Myeloproliferative Syndrome, Transient. The study of the Golgi Localization Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Golgi Localization Pathway has been researched in relation to Localization, Transport, Secretory, Secretion, Glycosylation. The Golgi Localization Pathway complements our catalog of research reagents including antibodies and ELISA kits against GOLPH3, GLYCOSYLTRANSFERASE, OSBP, GOLGA2, GOLGB1.

Top Research Reagents

We have 2394 products for the study of the Golgi Localization Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

H00010559-P01
12.5% SDS-PAGE Stained with Coomassie Blue.


Species Human
Applications WB, ELISA, PA

NB100-615
Western Blot: Caveolin-1 Antibody (7C8) [NB100-615] - Detection of caveolin in 3T3 cell lysates (50 ug). Lanes 1 and 2: 1:4000.  Lanes 3 and 4: 1:1000. Detection by ECL: 5 minute exposure.Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - Subcellular localization of mPARM-1 and hPARM-1 (full-length and mutant proteins). NIH/3T3 cells transiently expressing hPARM-1-GFP or deltaCT-GFP were fixed, immunostained for caveolin-1 (1:100, Novus Biologicals), and examined by confocal fluorescence microscopy. For hPARM-1-GFP-caveolin-1 co-localization, cells that  demonstrated cell membrane PARM-1 localization were chosen. All co-localizations were observed following merging images of GFP-tagged proteins with those of Golgi, endosomes, plasma membrane, alpha-tubulin or caveolin-1 labeling. Image collected and cropped by CiteAb from the following publication (https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-12-84), licensed under a CC-BY license.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, IB

     3 Reviews

37 Publications
NBP1-06274
Simple Western: GRP78/HSPA5 Antibody [NBP1-06274] - Image shows a specific band for GRP78 in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - C. elegans granulins impair organismal fitness and resistance to ER stress. Total worm lysates from synchronized day 1 adult granulin-expressing animals were immunoblotted with an anti-GRP78/HSPA5 antibody (3 biological replicates). Anti-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1008295), licensed under a CC-BY license.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, Flow

     2 Reviews

43 Publications
NBP1-31116
Western Blot: Caveolin-2 Antibody [NBP1-31116] -  Rat tissue extracts (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.Immunocytochemistry/Immunofluorescence: Caveolin-2 Antibody [NBP1-31116] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Caveolin 2 protein stained by Caveolin 2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

1 Publication
NBP1-49461
Analysis of endogenous STMN2 in mouse dorsal root ganglia (DRG) neurons.  A partial siRNA knockdown was used in the second lane. NBP1-49461 was used at a dilution of 1:1000. Image courtesy of Dr. Jung Eun Shin.Staining of STMN2 in primary mouse dorsal root ganglia (DRG) neurons Image shows the expected staining of endogenous STMN2 in axons and growth cones. Tubulin is a marker of axons. NBP1-49461 was used at a dilution of 1:4000. Image courtesy of Dr. Jung Eun Shin.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     2 Reviews

85 Publications
NBP1-50627
Knockout Validated: GOLM1 Antibody [NBP1-50627] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and GOLM1 knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:5,000 of Rabbit Anti-Human GOLM1 polyclonal Antibody (Catalog # NBP1-50627) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for GOLM1 at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.Western Blot: GOLM1 Antibody [NBP1-50627] - Western Blot Image of GOLM1. Whole cell protein from HeLa (lane 1), 3T3 (lane 2), MEF (lane 3), Raw264.7 (lane 4), L929 (lane 5), Neuro2A (lane 6) and PC12 (lane 7) were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 mg/ml anti-GOLM1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, KO

     3 Reviews

3 Publications
NBP1-40256
Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Hypoxia leads to EMT and ER-stress in CRC cells. B. C. Confluent growing SW480 (B) and HCT116 (C) cells were cultured under conditions of normoxia or hypoxia-like conditions (serum free; 100 uM CoCl2, 1-9 h. B-actin (B-act) as loading control. HIF1a was detectable after 3 h of CoCl2 incubation, the amount of the 50 kD-ATF6 fragment, was already enhanced after 1 h of addition of CoCl2. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0087386) licensed under a CC-BY license.Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Analysis of  ATF6 in mouse liver tissue using 3 ug/ml of ATF6 antibody and 0.25 ug/ml of GAPDH antibody.  Lane A contains 20 ugs of whole mouse liver lysate, lane B contains 20 ugs of total ER fraction, and lane C contains 20 ugs of rough ER fraction. The ATF6 band may represent under glycosylated or cleaved/active ATF6.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, IB

     3 Reviews

246 Publications
NBP1-80642
Immunocytochemistry/Immunofluorescence: UGT Antibody [NBP1-80642] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.Immunohistochemistry-Paraffin: UGT Antibody [NBP1-80642] - Staining of human Cerebral cortex shows moderate granular cytoplasmic positivity in neuronal and glial cells.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

1 Publication
NBP1-87789
Immunocytochemistry/Immunofluorescence: PKC mu Antibody [NBP1-87789] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.Immunohistochemistry-Paraffin: PKC mu Antibody [NBP1-87789] - Staining of human parathyroid gland shows cytoplasmic positivity in glandular cells.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

1 Publication
NBP2-00935
Western Blot: OSBP1 Antibody (1F2) [NBP2-00935] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBP1.Immunocytochemistry/Immunofluorescence: OSBP1 Antibody (1F2) [NBP2-00935] Staining of COS7 cells transiently transfected by pCMV6-ENTRY OSBP1.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

2 Publications
NBP2-22321
Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - HeLa cell lysate sample treated with DMSO was run on 8% SDS-PAGE. Both giantin monomer and  dimer are indicated. Western blot image submitted by a verified customer review.Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - PC-3 human prostate cancer cell line. Image was captured using EVOS M5000 microscope. Red - giantin; blue - nucleus, DAPI; bar 40 micron. ICC/IF image submitted by a verified customer review.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IP

     2 Reviews

6 Publications
NBP2-29906
Western Blot: ARF1 Antibody [NBP2-29906] - Analysis of 25ug of various whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel.Immunocytochemistry/Immunofluorescence: ARF1 Antibody [NBP2-29906] - Analysis of Arf1 in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with an Arf1 polyclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.

Rabbit Polyclonal
Species Human, Mouse, Canine
Applications WB, ICC/IF, IHC

2 Publications
AF5924
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5924) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=ST6 Gal Sialyltransferase 1/ST6GAL1 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5924) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (<a class=

Goat Polyclonal
Species Human
Applications WB, Simple Western, IHC

29 Publications
NBP2-34294
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Mouse thyroid tissue section stained with Thyroglobulin Antibody (2H11 + 6E1). IHC-P image submitted by a verified customer review.Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).

Mouse Monoclonal
Species Human, Mouse, Rat
Applications Flow, IHC, IHC-P

     3 Reviews

1 Publication
NBP2-53420
Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP2-53420 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-GM130/GOLGA2 Antibody NBP2-53420 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

16 Publications
NB200-111
Knockdown Validated: p14ARF/CDKN2A Antibody [NB200-111] - Inhibition of AKT decreases p53mut stability. T24 cells were transfected with non-targeting control, AKT1, or p14ARF siRNA. Cells were treated with NCS348884 (4 i1/4M), Nutlin3A (5 i1/4M) or DMSO as indicated. Whole cell lysates were probed with the indicated antibodies. Image collected and cropped by Citeab from the following publication (AKT regulates NPM dependent ARF localization and p53mut stability in tumors. <i>Oncotarget</i> (2014))  licensed under a CC-BY license.Immunohistochemistry: p14ARF/CDKN2A Antibody [NB200-111] - Inhibition of AKT modulates p53 stability in-vivo and synergizes with ionizing radiation to inhibit tumor growth( Sections of PSN1 xenografts treated with three consecutive doses of MK-2206 (60 mg/kg). Sections of PSN1 xenografts and in-vitro PSN1 cells fixed and stained with anti-NPM (red) and anti-p14ARF (green). Image collected and cropped by Citeab from the following publication (AKT regulates NPM dependent ARF localization and p53mut stability in tumors. Oncotarget (2014))  licensed under a CC-BY license.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

     2 Reviews

17 Publications
NBP2-75515
Immunocytochemistry/Immunofluorescence: GOLPH3 Antibody [NBP2-75515] - Staining GOLPH3 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Immunohistochemistry-Paraffin: GOLPH3 Antibody [NBP2-75515] - Analysis of paraffin-embedded rat epididymis tissue using anti-GOLPH3 antibody. Counter stained with hematoxylin.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF