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Cold Acclimation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Cold Acclimation Pathway and Physiological Stress, Dehydration, Obesity, Hypertrophy, Submersion. The study of the Cold Acclimation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Cold Acclimation Pathway has been researched in relation to Freezing Tolerance, Response To Cold, Transport, Photosynthesis, Secretion. The Cold Acclimation Pathway complements our catalog of research reagents including antibodies and ELISA kits against BAAT, CEBPZ, UCP1, RBPJ, TNFSF14.

Top Research Reagents

We have 2518 products for the study of the Cold Acclimation Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NB600-101
Knockdown Validated: Calreticulin Antibody [NB600-101] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1500 of Rabbit Anti-Human Calreticulin Polyclonal Antibody (Catalog # NB600-101) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Calreticulin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.Western Blot: Calreticulin Antibody [NB600-101] - Human kidney lysate.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, DB

28 Publications
NBP1-33427
Western Blot: csl/RBPJK Antibody [NBP1-33427] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with csl/RBPJK antibody [C1C3] (NBP1-33427) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody  was used to detect the primary antibody,Immunocytochemistry/Immunofluorescence: csl/RBPJK Antibody [NBP1-33427] -  Analysis of paraformaldehyde-fixed HeLa at 1:100 dilution.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, ChIP

     1 Review

1 Publication
NBP1-88543
Western Blot: FAM188A Antibody [NBP1-88543] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).Immunocytochemistry/Immunofluorescence: FAM188A Antibody [NBP1-88543] - Staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear membrane. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP2-11567
Western Blot: SLC6A8 Antibody [NBP2-11567] - Mouse Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/ml, 1 hr incubation. Detected by chemiluminescence.Immunohistochemistry-Paraffin: SLC6A8 Antibody [NBP2-11567] - Human kidney. Antibody at 5 ug/ml. Steamed antigen retrieval with citrate buffer pH6, AP-staining.

Goat Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

1 Publication
NBP2-15397
Western Blot: ANKRD1 Antibody [NBP2-15397] - Analysis of rat primary neonatal ventricular cardiomyocytes using ANKRD1 antibody. Image from verified customer review.Immunocytochemistry/Immunofluorescence: ANKRD1 Antibody [NBP2-15397] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF

1 Publication
AF2187
Western blot shows lysates of human brain (cerebellum) tissue and mouse brain (cerebellum) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Carbonic Anhydrase VIII/CA8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2187) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=    Simple  Western lane view shows lysates of human brain (cerebellum) tissue and mouse  brain (cerebellum) tissue, loaded at 0.2 mg/mL. Specific bands were  detected for Carbonic Anhydrase VIII/CA8 at approximately 41 and  50 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse  Carbonic Anhydrase VIII/CA8 Antigen Affinity-purified Polyclonal  Antibody (Catalog # AF2187) followed by 1:50 dilution of HRP-conjugated  Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Goat Polyclonal
Species Human, Mouse
Applications WB, Simple Western, IP

AF3398
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Catalase at approximately 62 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC

     3 Reviews

15 Publications
AF7197
Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and NMuMG mouse mammary gland epithelial cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Goat Polyclonal
Species Human, Mouse
Applications WB, IHC, ICC

11 Publications
MAB1417
Insulin was detected in immersion fixed  beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=Insulin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (<a class=

Rat Monoclonal
Species Human, Mouse, Bovine
Applications IHC, CyTOF-ready, ICC

24 Publications
MAB5826
Western blot shows lysates of human brain (cortex) tissue. PVDF Membrane was probed with 2 µg/mL of Human Cytosolic Sulfotransferase 4A1/SULT4A1 Monoclonal Antibody (Catalog # MAB5826) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=Cytosolic Sulfotransferase 4A1/SULT4A1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human Cytosolic Sulfotransferase 4A1/SULT4A1 Monoclonal Antibody (Catalog # MAB5826) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB, IHC

MAB4614
MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human Calcitonin R Monoclonal Antibody (Catalog # MAB4614, filled histogram) or isotype control antibody (Catalog # <a class=Calcitonin R knockout MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Calcitonin R Monoclonal Antibody (Catalog # MAB4614, filled histogram) or isotype control antibody (Catalog # <a class=

Mouse Monoclonal
Species Human
Applications Flow, CyTOF-ready, KO

6 Publications
MAB6158
Western blot shows recombinant human UCP1, recombinant human UCP2, recombinant human UCP3, and recombinant human UCP4 (5 ng/lane). PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=Western blot shows lysates of mouse brown adipose tissue and mouse adipose tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

Mouse Monoclonal
Species Human, Mouse
Applications WB, Simple Western, ICC

51 Publications
MAB1249
Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class= beta TC-6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249, filled histogram) or isotype control antibody (Catalog # <a class=

Mouse Monoclonal
Species Human, Mouse
Applications CyTOF-ready, ICC, ICFlow

     1 Review

13 Publications
664-LI
<P align=left>Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells. The ED<SUB>50</SUB> is 1-4 ng/mL.</P><p align=


Species Human
Applications BA

21 Publications
211-TBB/CF
Recombinant Human Lymphotoxin-alpha /TNF-beta  (Catalog # 211-TBB/CF) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 4‑20 pg/mL.1 µg/lane of Recombinant Human Lymphotoxin-alpha /TNF-beta  was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 19 kDa.


Species Human
Applications BA

8 Publications
NBP2-43648
Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Non-transfected (Negative) and transfected (Positive) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody  diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody  was used to detect the primary antibody.Immunocytochemistry/Immunofluorescence: CS Citrate Synthase Antibody (1761) [NBP2-43648] -  HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Citrate synthetase protein stained by Citrate synthetase antibody [1761] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

1 Publication
NBP2-47525
Western Blot: CBF2 Antibody [NBP2-47525] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10<br/>Lane 2: Human cell line NTERA-2Immunohistochemistry: CBF2 Antibody [NBP2-47525] - Analysis of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

     2 Reviews

NB100-56503
Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Metformin activates apoptotic cell death; Right panel: MCF-7 cells were kept in the presence or absence of metformin for 20 days and then processed to obtain mitochondrial or whole cellular extracts. BAX and CYCS expression levels were determined by Western blot as indicated under Material and Methods. Densitometric analysis of the gels was performed as indicated under Material and Methods. PHB and CDK4 were used as purity and loading controls. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2073-4409/8/1/49), licensed under a CC-BY license.Immunocytochemistry/Immunofluorescence: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - p73 was detected in immersion fixed Hela human cell line using  NB100-56503 at 25 ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Staining was observed in the cytoplasm and mitochondria.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, Flow

61 Publications
NBP2-92503
Western Blot: BAAT Antibody [NBP2-92503] - Analysis of extracts of various cell lines, using BAAT at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.Immunocytochemistry/Immunofluorescence: BAAT Antibody [NBP2-92503] - Analysis of Hep G2 cells using BAAT Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

NBP2-93416
Western Blot: SLC10A2 Antibody [NBP2-93416] - Mouse liver cell lysate probed with SLC10A2 antibody. WB image submitted by a verified customer review.Western Blot: SLC10A2 Antibody [NBP2-93416] - Analysis of extracts of various cell lines, using SLC10A2 at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB

     1 Review