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Axis Specification Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Axis Specification Pathway and Nervousness, Kyphosis Deformity Of Spine, Overlying Behavior, Tissue Adhesions, Hypoxia. The study of the Axis Specification Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Axis Specification Pathway has been researched in relation to Localization, Gastrulation, Oogenesis, Embryonic Axis Specification, Transport. The Axis Specification Pathway complements our catalog of research reagents including antibodies and ELISA kits against WNT, BETA-CATENIN, AP, GRK, CHORDIN.

Top Research Reagents

We have 860 products for the study of the Axis Specification Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

Activin RIB/ALK-4 Antibody (3U6I8)
NBP3-16106
Western Blot: Activin RIB/ALK-4 Antibody (3U6I8) [NBP3-16106] - Western blot analysis of extracts of various cell lines, using Activin RIB/ALK-4 Rabbit mAb (NBP3-16106) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Immunohistochemistry-Paraffin: Activin RIB/ALK-4 Antibody (3U6I8) [NBP3-16106] - Immunohistochemistry of paraffin-embedded mouse brain using Activin RIB/ALK-4 Rabbit mAb (NBP3-16106) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

LEF1 Antibody (3H5)
H00051176-M01
Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - LEF1 monoclonal antibody (M01), clone 3H5 Analysis of LEF1 expression in MES-SA/Dx5.Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - Analysis of LEF1 over-expressed 293 cell line, cotransfected with LEF1 Validated Chimera RNAi ( Cat # H00051176-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with LEF1 monoclonal antibody (M01), clone 3H5 (Cat # H00051176-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Mouse Monoclonal
Species Human, Mouse
Applications WB, ELISA, ICC/IF

3 Publications
CRIPTO Antibody - BSA Free
NB100-1598
Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.Immunocytochemistry/Immunofluorescence: CRIPTO Antibody [NB100-1598] - CRIPTO antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Rabbit Polyclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

3 Publications
HNF-4 alpha/NR2A1 Antibody
NBP1-89679
Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining in human duodenum and tonsil tissues using anti-HNF4A antibody. Corresponding HNF4A RNA-seq data are presented for the same tissues.Western Blot: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Analysis in human cell lines Caco-2 and PC-3 using anti-HNF4A antibody. Corresponding HNF4A RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

4 Publications
Axin-1 Antibody
AF3287
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Axin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3287) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB

15 Publications
Wnt-8a Antibody [Unconjugated]
AF2248

Goat Polyclonal
Species Mouse
Applications WB, IHC

3 Publications
Smad2/3 Antibody [Unconjugated]
AF3797
Western blot shows lysates of A549 human lung carcinoma cell line, Jurkat human acute T cell leukemia cell line, HEK293 human embryonic kidney cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Smad2/3/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) or control antibody (Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Human, Mouse
Applications WB, Simple Western, IHC

     1 Review

24 Publications
TCF7L1/TCF3 Antibody
AF6116
Western blot shows lysates of human pancreas and human lung tissue. PVDF Membrane was probed with 0.2 µg/mL of Human TCF7L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6116) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=TCF7L1 was detected in immersion fixed BG01V human embryonic stem cells using Human TCF7L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6116) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, upper panel; Catalog # <a class=

Sheep Polyclonal
Species Human
Applications WB, ICC

2 Publications
PITX2 Antibody [Unconjugated]
AF7388
PITX2 was detected in immersion fixed paraffin-embedded sections of human thyroid cancer tissue using Sheep Anti-Human PITX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7388) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

Sheep Polyclonal
Species Human
Applications IHC

8 Publications
TCF-3/E2A Antibody (826927) [Unconjugated]
MAB7650
Western blot shows lysates of D3 mouse embryonic stem cell line and P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse TCF-3/E2A Monoclonal Antibody (Catalog # MAB7650) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=TCF‑3/E2A was detected in immersion fixed frozen sections of E9.5 mouse embryo using Rat Anti-Mouse TCF‑3/E2A Monoclonal Antibody (Catalog # MAB7650) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=

Rat Monoclonal
Species Mouse
Applications WB, IHC

2 Publications
Lefty-1 Antibody (146903)
MAB994

Rat Monoclonal
Species Mouse
Applications WB

1 Publication
Nodal [Unconjugated]
3218-ND


Species Human
Applications BA

22 Publications
BMP-4 [Unconjugated]
314-BP
Recombinant Human BMP‑4 (Catalog # 314-BP) induces BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED<sub>50</sub> for this effect is 0.70-7.00 ng/mL.1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in gel are due to variable glycosylation.<p style=


Species Human
Applications BA, BA

519 Publications
EGF [Unconjugated]
236-EG
Recombinant Human EGF Protein (Catalog # 236-EG) has a molecular weight (MW) of 6.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED<sub>50</sub> for this effect is 20‑100 pg/mL.


Species Human
Applications BA

835 Publications
DSPP Antibody - Azide and BSA Free
NBP2-92546
Western Blot: DSPP Antibody [NBP2-92546] - Analysis of lysates from wild type (WT) and 293F cells transfected with DSPP using DSPP Rabbit pAb at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution.Lysates/proteins: 40.5 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit. Exposuretime: 10s.ICC/IF:DSPP Antibody [NBP2-92546] - Analysis of Dental pulp stem cell using DSPP Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ELISA, ICC/IF

3 Publications