| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80885 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73049 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73045 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-74033-50ul | |||
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Antioxidant enzyme synthesis in response to orange oil treatment. Immunoblot analysis demonstrating expression of NQO1 protein at 6, 12 and 24 hrs following 15 min treatment of BEAS-2B cells with the oil preparation or time-matched soy oil control. Representative blots from one of three separate experiments are shown above. Densitometric evaluations of each target protein blot normalized to its corresponding GAPDH for all three experiments are provided below. Bars represent mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-12-92), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Western-Blot-NB200-209-img0019.jpg "<b>Biological Strategies Validation. </b>Western Blot: NQO-1 Antibody (A180) [NB200-209] - Antioxidant enzyme synthesis in response to orange oil treatment. Immunoblot analysis demonstrating expression of NQO1 protein at 6, 12 and 24 hrs following 15 min treatment of BEAS-2B cells with the oil preparation or time-matched soy oil control. Representative blots from one of three separate experiments are shown above. Densitometric evaluations of each target protein blot normalized to its corresponding GAPDH for all three experiments are provided below. Bars represent mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-12-92), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NQO-1 Antibody (A180) [NB200-209] - NQO1 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Immunocytochemistry-Immunofluorescence-NB200-209-img0010.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Analysis of NQO1 in human breast cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Immunohistochemistry-Paraffin-NB200-209-img0014.jpg)
![Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U-87 cells with NQO-1 [A180] Antibody NB200-209AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Flow-Cytometry-NB200-209-img0018.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Detection of NQO1 in A549 lysates using NB 200-209. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Western-Blot-NB200-209-img0007.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Immunohistochemistry showing Anti-NQ01 on formalin fixed paraffin embedded human kidney tissue](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Immunohistochemistry-Paraffin-NB200-209-img0012.jpg)
![Flow (Intracellular): NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U87MG cells with NQO1 (A180) antibody NBP2-24917 (blue) along with a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Flow-Intracellular-NB200-209-img0015.jpg)
![Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - Detection of NQO-1 in Human A549 Cell Line by Flow Cytometry. Human A549 cell line was stained with Mouse Anti- NQO-1 Monoclonal Antibody (Catalog # NB200-209, filled histogram), or Mouse IgG1 isotype control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Flow-Cytometry-NB200-209-img0016.jpg)
![Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U-87 cells with NQO-1 [A180] Antibody NB200-209C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Flow-Cytometry-NB200-209-img0017.jpg)
![Simple Western: NQO-1 Antibody (A180) [NB200-209] - Simple Western lane view shows a specific band for NQO-1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Simple-Western-NB200-209-img0013.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Antioxidant enzyme synthesis in response to orange oil treatment. Immunoblot analysis demonstrating expression of HO-1, NQO1 & GCLm proteins at 6, 12 & 24 hrs following 15 min treatment of BEAS-2B cells with the oil preparation or time-matched soy oil control. Representative blots from one of three separate experiments are shown above. Densitometric evaluations of each target protein blot normalized to its corresponding GAPDH for all three experiments are provided below. Bars represent mean ± SEM. Image collected & cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-12-92), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202415175255.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Equol attenuated ER stress by activating Nrf2 in vitro & in vivo.(A) HUVECs were incubated with different concentrations (1, 10 & 100 nM) of equol for 24 h before treatment with t-BHP (50 μM) for another 6 h. The expression of Nrf2 & NQO1 was detected by western blotting. (C) Total tissue lysates from the thoracic & abdominal aorta of apoE-/- mice with or without equol treatment were immunoblotted with anti-Nrf2 & anti-beta -actin antibodies. (E) Cells were transfected with Nrf2 siRNA for 5~6 h; the medium was then replaced with fresh culture medium, followed by incubation for another 24 h. Thereafter, the cells were treated with equol (100 nM) for 24 h & then were incubated with t-BHP (50 μM) for an additional 6 h. The cells were collected & lysed, & western blot analysis was performed. (B)(D)(F) The bar charts show the quantification of the indicated proteins. (G) HUVECs were transfected with Nrf2 siRNA & treated as described in (C), & cell apoptosis was assayed using the Cell Death Detection ELISAplus Kit. Values are presented as means ± SD. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001 versus the control group, RC group or siRNA treated with no drug group; #p < 0.01, ##p < 0.001 versus the t-BHP-treated group & siRNA plus t-BHP treated group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27907038), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416212320.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - S-(-)equol primarily up-regulated Nrf2 protein expression activating ARE-dependent response.(A) Whole EA.hy926 cells lysates of cells treated with 250 nM S-(-)equol, 500 nM daidzein, or 50 µM tBHQ for 24 h were subjected to immunoblot analysis with anti-Nrf2, anti-HO-1, anti-NQO1, & anti-beta -tubulin antibodies. (B) Total HUVECs lysates of cell treated with 250 nM S-(-)equol for 24 h were immunoblotted with anti-Nrf2 & anti-beta -tubulin antibodies. (C) EA.hy926 cells were transfected with plasmids containing an HA-tagged Nrf2 open reading frame & the renilla firefly luciferase gene. Medium was changed after 6 hours of incubation. Where indicated, extracts from HA-Nrf2 EA hy.92 cells were additionally probed with anti-HA antibody, anti-Nrf2, anti-HO-1, anti-NQO1 & anti-beta -tubulin antibodies. Values are means of three independent experiments with standard deviations represented by vertical bars. Mean values were significantly different compared to controls (*p<0.05). Total RNA was extracted from EA.hy926 cells or HUVECs treated as indicated, & Nrf2 (D), HO-1 (E), & NQO1 (F) mRNA was measured by real-time RT-PCR analysis. The values shown represent the mean ± SD obtained for three independent experiments (*p<0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24260155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416205135.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - S-(-)equol primarily up-regulated Nrf2 protein expression activating ARE-dependent response.(A) Whole EA.hy926 cells lysates of cells treated with 250 nM S-(-)equol, 500 nM daidzein, or 50 µM tBHQ for 24 h were subjected to immunoblot analysis with anti-Nrf2, anti-HO-1, anti-NQO1, & anti-beta -tubulin antibodies. (B) Total HUVECs lysates of cell treated with 250 nM S-(-)equol for 24 h were immunoblotted with anti-Nrf2 & anti-beta -tubulin antibodies. (C) EA.hy926 cells were transfected with plasmids containing an HA-tagged Nrf2 open reading frame & the renilla firefly luciferase gene. Medium was changed after 6 hours of incubation. Where indicated, extracts from HA-Nrf2 EA hy.92 cells were additionally probed with anti-HA antibody, anti-Nrf2, anti-HO-1, anti-NQO1 & anti-beta -tubulin antibodies. Values are means of three independent experiments with standard deviations represented by vertical bars. Mean values were significantly different compared to controls (*p<0.05). Total RNA was extracted from EA.hy926 cells or HUVECs treated as indicated, & Nrf2 (D), HO-1 (E), & NQO1 (F) mRNA was measured by real-time RT-PCR analysis. The values shown represent the mean ± SD obtained for three independent experiments (*p<0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24260155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-31020241620372.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Phf2 protects the liver from high fat & high sucrose diet-induced fibrogenesis during obesity. GFP or Phf2 were overexpressed through AAV strategy specifically in the liver of 7-week-old male C57Bl/6 J mice. Mice were then fed with either a chow diet (NCD) or with a high fat & high sucrose diet (HFHSD) for 16 weeks. Mice were studied in the fed state. a Representative western blot analysis showing the contribution of Phf2 overexpression to the regulation of the PI3K/Akt signaling & pro-fibrogenic pathways in liver extracts (n = 15 per group). b (Left) Oral glucose tolerance test & insulin levels during the OGTT test. (right) Insulin tolerance test (n = 15 per group). c Liver sections stained with hematoxylin & eosin (H&E), trichrome masson & sirius red are shown. Scale bars = 100 μm (n = 10 per group). d Percentage of fibrotic area & percentage of apoptotic hepatocytes (n = 15 per group). e Relative oxidized GSSG content & measurement of Gpx activity (n = 10 per group). f Levels of carbonylated proteins (n = 10 mice per group). All error bars represent mean ± SEM. Statistical analyses were made using unpaired t-test (b) or Anova, followed by Bonferonni’s test (d, e). *P < 0.01 HFHSD/GFP compared to HFHSD/Phf2, $P < 0.01 HFHSD/GFP compared to NCD/GFP, £P < 0.01 HFHSD/Phf2 compared to HFHSD/GFP Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04361-y), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-31020241621823.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Activation of Nrf2 signaling pathway by FST in RAW 264.7 mouse macrophage-like cells. Cells were treated with FST with or without 100 ng/mL RANKL for 5 days. (A) Total cellular proteins were isolated from cells & the expression levels of Nrf2 & its regulatory proteins were assessed by Western blot analysis. beta -actin was used as the internal control. (C) The expression of nuclear & cytosol Nrf2 were determined by Western blotting. Lamin B & beta -actin were used as internal controls for the nuclear & cytosolic fractions, respectively. The results shown are representative of three independent experiments. (B,D) Statistical analyses were conducted using analysis of variances between groups. * p < 0.05 & *** p < 0.0001 when compared to control. # p < 0.05 & ### p < 0.0001 when compared to RANKL treatment. RANKL: receptor of activator of nuclear factor kappa-B ligand; FST: fermented sea tangle extract; Nrf2: nuclear factor-erythroid 2-related factor 2; p-Nrf2: phosphorylated nuclear factor-erythroid 2-related factor 2; HO-1: heme oxygenase-1; NQO-1: NAD(P)H quinone oxidoreductase 1; +: cells treated the reagent; -: cells untreated the reagent. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31357503), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416205186.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Nrf2-pathway activation by hybrids: nuclear translocation & targets induction. (A) Nuclear cellular extracts of SH-SY5Y cells were treated for 3 hours with compounds at 5 µM, 500 nM, & 50 nM or with 20, 10, & 5 µM dimethyl fumarate (DMF). Nrf2 protein content in the nucleus was determined by Western blot. Anti-lamin A/C was used as a protein loading control. Results are shown as ratio Nrf2/lamin A/C ± SEM; *p < 0.05, **p < 0.01 & ****p < 0.0001 versus CTR; Dunnett’s multiple comparison test (F ratio = 6.797, n≥3). (B–C) RNA from total cellular extracts of SH-SY5Y cells, treated for 24 hours with 5 μM compounds or 20 μM DMF, were analyzed for NQO1 (B) & HO-1 (C) mRNA expression by RT-qPCR. GAPDH was used as housekeeping gene. Results are shown as mean ± SEM; *p < 0.05, **p < 0.01, & ***p < 0.001 versus CTR; Dunnett’s multiple comparison test (B, n≥3, F ratio = 10.44; C, n≥3, F ratio = 13.95). (D–E) Cellular extracts of SH-SY5Y cells treated for 24 hours with compounds at 5 μM or 20 μM DMF were analyzed for NQO1 (D) & HO-1 (E) protein levels by Western blot. Anti-actin was used as protein loading control. Results are shown as ratio (% of CTR) ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, & ****p < 0.0001 versus CTR; Dunnett’s multiple comparison test (D, n ≥ 3, F ratio = 5.144; E, n≥3, F ratio = 17.26). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32047434), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416205137.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - The effects of PI3K/Akt & ER inhibitors on S-(-)equol-induced Nrf2 activation in endothelial cells.(A) EA.hy926 cells or HUVECs transfected w/ ARE-dependent firefly luciferase reporter gene (F) & the renilla firefly luciferase gene (R), & treated w/ 250 nM S-(-)equol in the presence or absence of 10 µM LY294002 or 100 nM ICI182,780 for 16 h. The potency of induction is expressed as the relative luminescence (R/F) measured using the dual luciferase reporter assay system (mean ± SD, n = 3). *p<0.05 versus w/ control group, #p<0.05 versus w/ S-(-)equol treated group. (B) EA.hy926 cells or HUVECs incubated w/ or w/out 10 µM LY294002 or 100 nM ICI182,780 for 30 min & then w/ or w/out 250 nM S-(-)equol or 500 nM daidzein for 24 h. Whole cell lysates then immunoblotted w/ antibodies against Nrf2, HO-1, NQO1, & beta -tubulin. Values are means of three independent experiments w/ standard deviations represented by vertical bars. Mean values significantly different compared w/ controls (*p<0.05). (C) EA.hy926 cells or HUVECs cotransfected w/ the HA-Nrf2 & renilla firefly luciferase expression plasmids treated w/ 250 nM S-(-)equol in the presence or absence of 10 µM LY294002 or 100 nM ICI182,780 for 16 h, & HA-Nrf2 localization analyzed by confocal microscopy. (D) EA.hy926 cells incubated w/ 250 nM S-(-)equol, 500 nM daidzein w/ or w/out 10 µM LY294002 or 100 nM ICI182,780 for 16 h & then immunostained w/ an antibody against Nrf2, & Nrf2 localization analyzed by confocal microscopy. Values (intensity of nuclear versus cytoplasmic) are means of counting 100 cells w/ standard deviations represented by vertical bars. *p<0.05 versus w/ control group, #p<0.05 versus w/ S-(-)equol treated group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24260155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416205110.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - S-(-)equol primarily up-regulated Nrf2 protein expression activating ARE-dependent response.(A) Whole EA.hy926 cells lysates of cells treated with 250 nM S-(-)equol, 500 nM daidzein, or 50 µM tBHQ for 24 h were subjected to immunoblot analysis with anti-Nrf2, anti-HO-1, anti-NQO1, & anti-beta -tubulin antibodies. (B) Total HUVECs lysates of cell treated with 250 nM S-(-)equol for 24 h were immunoblotted with anti-Nrf2 & anti-beta -tubulin antibodies. (C) EA.hy926 cells were transfected with plasmids containing an HA-tagged Nrf2 open reading frame & the renilla firefly luciferase gene. Medium was changed after 6 hours of incubation. Where indicated, extracts from HA-Nrf2 EA hy.92 cells were additionally probed with anti-HA antibody, anti-Nrf2, anti-HO-1, anti-NQO1 & anti-beta -tubulin antibodies. Values are means of three independent experiments with standard deviations represented by vertical bars. Mean values were significantly different compared to controls (*p<0.05). Total RNA was extracted from EA.hy926 cells or HUVECs treated as indicated, & Nrf2 (D), HO-1 (E), & NQO1 (F) mRNA was measured by real-time RT-PCR analysis. The values shown represent the mean ± SD obtained for three independent experiments (*p<0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24260155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416212388.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Activation of Nrf2 signaling pathway by FST in RAW 264.7 mouse macrophage-like cells. Cells were treated with FST with or without 100 ng/mL RANKL for 5 days. (A) Total cellular proteins were isolated from cells & the expression levels of Nrf2 & its regulatory proteins were assessed by Western blot analysis. beta -actin was used as the internal control. (C) The expression of nuclear & cytosol Nrf2 were determined by Western blotting. Lamin B & beta -actin were used as internal controls for the nuclear & cytosolic fractions, respectively. The results shown are representative of three independent experiments. (B,D) Statistical analyses were conducted using analysis of variances between groups. * p < 0.05 & *** p < 0.0001 when compared to control. # p < 0.05 & ### p < 0.0001 when compared to RANKL treatment. RANKL: receptor of activator of nuclear factor kappa-B ligand; FST: fermented sea tangle extract; Nrf2: nuclear factor-erythroid 2-related factor 2; p-Nrf2: phosphorylated nuclear factor-erythroid 2-related factor 2; HO-1: heme oxygenase-1; NQO-1: NAD(P)H quinone oxidoreductase 1; +: cells treated the reagent; -: cells untreated the reagent. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31357503), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416203710.jpg)
![Western Blot: NQO-2 Antibody [NBP1-31563] - Sample(30 ug whole cell lysate)A:293T B:A431 12% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/NQO-2-Antibody-Western-Blot-NBP1-31563-img0011.jpg "Western Blot: NQO-2 Antibody [NBP1-31563] - Sample(30 ug whole cell lysate)A:293T B:A431 12% SDS PAGE, antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: NQO-2 Antibody [NBP1-31563] - PLC xenograft, using NQO2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NQO-2-Antibody-Immunohistochemistry-Paraffin-NBP1-31563-img0009.jpg)
![Western Blot: NQO-2 Antibody [NBP1-31563] - Sample (50 ug of whole cell lysate) A: Mouse liver 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/NQO-2-Antibody-Western-Blot-NBP1-31563-img0010.jpg)
![Western Blot: NQO2 Antibody (3G4) [NBP2-02062] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-NQO2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/NQO2-Antibody-3G4-Western-Blot-NBP2-02062-img0009.jpg "Western Blot: NQO2 Antibody (3G4) [NBP2-02062] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-NQO2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry-Paraffin: NQO2 Antibody (3G4) [NBP2-02062] - Carcinoma of Human bladder tissue.](http://images.novusbio.com/fullsize/NQO2-Antibody-3G4-Immunohistochemistry-Paraffin-NBP2-02062-img0010.jpg)
![Western Blot: NQO2 Antibody (3G4) [NBP2-02062] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NQO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NQO2.](http://images.novusbio.com/fullsize/NQO2-Antibody-3G4-Western-Blot-NBP2-02062-img0001.jpg)
![Immunohistochemistry-Paraffin: NQO2 Antibody (3G4) [NBP2-02062] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NQO2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NQO2-Antibody-3G4-Immunohistochemistry-Paraffin-NBP2-02062-img0002.jpg)
![Immunohistochemistry-Paraffin: NQO2 Antibody (3G4) [NBP2-02062] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NQO2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NQO2-Antibody-3G4-Immunohistochemistry-Paraffin-NBP2-02062-img0003.jpg)
![Immunohistochemistry-Paraffin: NQO2 Antibody (3G4) [NBP2-02062] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NQO2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NQO2-Antibody-3G4-Immunohistochemistry-Paraffin-NBP2-02062-img0004.jpg)
![Immunohistochemistry-Paraffin: NQO2 Antibody (3G4) [NBP2-02062] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NQO2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NQO2-Antibody-3G4-Immunohistochemistry-Paraffin-NBP2-02062-img0005.jpg)
![Immunohistochemistry-Paraffin: NQO2 Antibody (3G4) [NBP2-02062] - Staining of paraffin-embedded Human Kidney tissue using anti-NQO2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NQO2-Antibody-3G4-Immunohistochemistry-Paraffin-NBP2-02062-img0006.jpg)
![Immunohistochemistry-Paraffin: NQO2 Antibody (3G4) [NBP2-02062] - Staining of paraffin-embedded Human pancreas tissue using anti-NQO2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NQO2-Antibody-3G4-Immunohistochemistry-Paraffin-NBP2-02062-img0007.jpg)
![Immunohistochemistry-Paraffin: NQO2 Antibody (3G4) [NBP2-02062] - Staining of paraffin-embedded Human prostate tissue using anti-NQO2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NQO2-Antibody-3G4-Immunohistochemistry-Paraffin-NBP2-02062-img0008.jpg)
![Western Blot: NQO-2 Antibody [NBP1-85221] - Analysis in control (vector only transfected HEK293T lysate) and NQO2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/NQO-2-Antibody-Western-Blot-NBP1-85221-img0012.jpg "Western Blot: NQO-2 Antibody [NBP1-85221] - Analysis in control (vector only transfected HEK293T lysate) and NQO2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: NQO-2 Antibody [NBP1-85221] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol.](http://images.novusbio.com/fullsize/NQO-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85221-img0011.jpg)
![Immunohistochemistry-Paraffin: NQO-2 Antibody [NBP1-85221] - Staining of human urinary bladder shows low expression as expected.](http://images.novusbio.com/fullsize/NQO-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85221-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NQO-2 Antibody [NBP1-85221] - Analysis in human kidney and urinary bladder tissues. Corresponding NQO2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NQO-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85221-img0013.jpg)
![Immunohistochemistry-Paraffin: NQO-2 Antibody [NBP1-85221] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/NQO-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85221-img0014.jpg)
![Immunohistochemistry-Paraffin: NQO-2 Antibody [NBP1-85221] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/NQO-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85221-img0016.jpg)
![Western Blot: NQO-2 Antibody [NBP1-85222] - Analysis in control (vector only transfected HEK293T lysate) and NQO2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/NQO-2-Antibody-Western-Blot-NBP1-85222-img0009.jpg "Western Blot: NQO-2 Antibody [NBP1-85222] - Analysis in control (vector only transfected HEK293T lysate) and NQO2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NQO-2 Antibody [NBP1-85222] - Staining in human kidney and pancreas tissues using anti-NQO2 antibody. Corresponding NQO2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NQO-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85222-img0008.jpg)
![Immunohistochemistry-Paraffin: NQO-2 Antibody [NBP1-85222] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/NQO-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85222-img0005.jpg)
![Immunohistochemistry-Paraffin: NQO-2 Antibody [NBP1-85222] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/NQO-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85222-img0006.jpg)
![Western Blot: NQO-2 Antibody (3C11) [NBP2-46211] - Analysis of extracts (35ug) from 9 different cell line (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/NQO-2-Antibody-3C11-Western-Blot-NBP2-46211-img0002.jpg "Western Blot: NQO-2 Antibody (3C11) [NBP2-46211] - Analysis of extracts (35ug) from 9 different cell line (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Western Blot: NQO-2 Antibody (3C11) [NBP2-46211] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NQO-2.](http://images.novusbio.com/fullsize/NQO-2-Antibody-3C11-Western-Blot-NBP2-46211-img0001.jpg)
![Western Blot: NQO-2 Antibody (1E3) [NBP1-74033] - Mouse liver lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NQO2 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/NQO-2-Antibody-1E3-Western-Blot-NBP1-74033-img0002.jpg "Western Blot: NQO-2 Antibody (1E3) [NBP1-74033] - Mouse liver lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NQO2 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Western Blot: NQO-2 Antibody (5E0B2) [NBP3-16284] - Western blot analysis of extracts of various cell lines, using NQO-2 Rabbit mAb (NBP3-16284) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/NQO-2-Antibody-5E0B2-Western-Blot-NBP3-16284-img0001.jpg "Western Blot: NQO-2 Antibody (5E0B2) [NBP3-16284] - Western blot analysis of extracts of various cell lines, using NQO-2 Rabbit mAb (NBP3-16284) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: NQO-2 Antibody (3E8-G2) [H00004835-M01] - Analysis of NQO2 expression in transfected 293T cell line by NQO2 monoclonal antibody (M01), clone 3E8-G2.Lane 1: NQO2 transfected lysate(26 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NQO-2-Antibody-3E8-G2-Western-Blot-H00004835-M01-img0003.jpg "Western Blot: NQO-2 Antibody (3E8-G2) [H00004835-M01] - Analysis of NQO2 expression in transfected 293T cell line by NQO2 monoclonal antibody (M01), clone 3E8-G2.Lane 1: NQO2 transfected lysate(26 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: NQO-2 Antibody (3E8-G2) [H00004835-M01] - Detection limit for recombinant GST tagged NQO2 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NQO-2-Antibody-3E8-G2-Sandwich-ELISA-H00004835-M01-img0002.jpg)
![Western Blot: NQO-2 Antibody (8E12) [NBP3-26458] - Positive Western Blot detected in: HepG2 whole cell lysate, Ntera-2 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, Mouse liver tissue. All lanes: NQO-2 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 26 kDa Observed band size: 26 kDa](http://images.novusbio.com/fullsize/nbp3-26458_rabbit-nqo-2-mab-8e12-28220241135163.jpg "Western Blot: NQO-2 Antibody (8E12) [NBP3-26458] - Positive Western Blot detected in: HepG2 whole cell lysate, Ntera-2 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, Mouse liver tissue. All lanes: NQO-2 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 26 kDa Observed band size: 26 kDa")
![Western Blot: NQO2 Overexpression Lysate (Adult Normal) [NBL1-13763] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NQO2.](http://images.novusbio.com/fullsize/NQO2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13763-img0002.jpg "Western Blot: NQO2 Overexpression Lysate (Adult Normal) [NBL1-13763] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NQO2.")
![SDS-Page: Recombinant Human NQO-2 His Protein [NBP1-41152] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Human-NQO-2-His-Protein-SDS-Page-NBP1-41152-img0001.jpg "SDS-Page: Recombinant Human NQO-2 His Protein [NBP1-41152] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")