| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77397SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77396SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34671-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44754 | |||
| Azide and BSA Free | NBP2-34670-0.1mg | |||
| Azide and BSA Free | NBP2-34670-0.2mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47843-0.1mg | |||
| IHC-Prediluted | NBP2-48187 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70967 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37617-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44755 | |||
| Azide and BSA Free | NBP2-34734-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44757 | |||
| Azide and BSA Free | NBP2-34625-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47844-0.1mg | |||
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0015.jpg "Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: HSP60 Antibody [NBP1-77397] - Analysis of extracts from HeLa and COS cells using NBP1-77397 Hsp60 antibody at 1:1000](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP1-77397-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody - BSA Free [NBP1-77397] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0019.jpg)
![Immunohistochemistry: HSP60 Antibody [NBP1-77397] - Staining of HSP60 in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunohistochemistry-NBP1-77397-img0006.jpg)
![Flow Cytometry: HSP60 Antibody [NBP1-77397] - Analysis of HSP60 in RM1 cells (murine prostate cancer cell line) using anti-HSP60 antibody. The primary antibody was used at a dilution of 1:100. Image from verified customer review.](http://images.novusbio.com/fullsize/HSP60-Antibody-Flow-Cytometry-NBP1-77397-img0014.jpg)
![Western Blot: HSP60 Antibody [NBP1-77397] - Analysis of HSP60 in: 1) HeLa, 2) HepG2, 3) NIH/3T3, 4) Jurkat, 5) CHO, 6) A431, 7) PC12 and 8) COS7](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP1-77397-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HSP60 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - Confocal immunofluorescence analysis of HeLa cells using Hsp60 antibody at 1:50 (green). Nuclei were counterstained using DAPI (blue).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - Confocal immunofluorescent analysis of HeLa cells using Hsp60 antibody (NBP1-77397, 1:100). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody conjugated to Alexa Fluor 488 (NBP1-77397AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody - BSA Free [NBP1-77397] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody - BSA Free [NBP1-77397] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0018.jpg)
![Simple Western: HSP60 Antibody [NBP1-77397] - Lane view shows a specific band for Hsp60 in 0.05 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP60-Antibody-Simple-Western-NBP1-77397-img0013.jpg)
![Western Blot: HSP60 Antibody [NBP1-77396] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP1-77396-img0007.jpg "Western Blot: HSP60 Antibody [NBP1-77396] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77396] - Hsp60 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77396-img0004.jpg)
![Immunohistochemistry: HSP60 Antibody [NBP1-77396] - IHC staining of HSP60 in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunohistochemistry-NBP1-77396-img0006.jpg)
![Flow (Intracellular): HSP60 Antibody [NBP1-77396] - An intracellular stain was performed on HeLa cells with HSP60 Antibody NBP1-77396AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/HSP60-Antibody-Flow-Intracellular-NBP1-77396-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77396] - Antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77396-img0005.jpg)
![Simple Western: HSP60 Antibody [NBP1-77396] - Image shows a specific band for Hsp60 in 0.05 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP60-Antibody-Simple-Western-NBP1-77396-img0009.jpg)
![Simple Western: HSP60 Antibody (LK2) [NBP2-32867] - Simple Western lane view shows a specific band for HSP60 in 0.2 mg/ml of HeLa (left) and HepG2 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Simple-Western-NBP2-32867-img0002.jpg "Simple Western: HSP60 Antibody (LK2) [NBP2-32867] - Simple Western lane view shows a specific band for HSP60 in 0.2 mg/ml of HeLa (left) and HepG2 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: HSP60 Antibody (LK2) [NBP2-32867] - Western blot analysis of Hep2 cell lysate using HSP60 Mouse Monoclonal Antibody (LK2).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Western-Blot-NBP2-32867-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (LK2) [NBP2-32867] - Immunofluorescent staining of MEOH-fixed HeLa cells using followed by goat anti-Mouse labeled with CF488 (Green); Nuclei are labeled with NucSpot red).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Immunocytochemistry-Immunofluorescence-NBP2-32867-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (LK2) [NBP2-32867] - Formalin-fixed, paraffin-embedded human liver in colon stained with HSP60 Mouse Monoclonal Antibody (LK2).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Immunohistochemistry-Paraffin-NBP2-32867-img0008.jpg)
![Western Blot (Negative): HSP60 Antibody (LK2) [NBP2-32867] - Immunoblot analysis of whole bacterial lysate of Mtb cells pretreated with (+) or without (-) proteinase K followed by incubation with K632-7 poly-Ub chains at 4C for 4 hr. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-09955-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Western-Blot-Negative-NBP2-32867-img0006.jpg)
![Simple Western: HSP60 Antibody (LK2) [NBP2-32867] - Electropherogram image of the corresponding Simple Western lane. HSP60 antibody was used at 10 ug/ml dilution of HeLa and HepG2 lysates(s) respectively.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Simple-Western-NBP2-32867-img0004.jpg)
![Western Blot: HSP60 Antibody (LK2) [NBP2-32867] - Mtb Rv1468c directly binds to poly-Ub chains in a UBA-dependent manner. a, b Immunoblot analysis of whole bacterial lysate of Mtb cells pretreated w/ (+) or w/out (–) proteinase K followed by incubation w/ K632–7 (a) or K482–7 (b) poly-Ub chains at 4 °C for 4 h. c Confocal microscopy analysis for colocalization of pSC301-Mtb stably expressing green fluorescent protein (GFP) w/ K632–7 or K482–7 Ub chains in vitro. Bacteria (green) treated w/ (+) or w/out (–) proteinase K further incubated w/ poly-Ub chains as in a & b, & then immunostained using anti-Ub antibody (red). Scale bars, 10 μm. The % colocalizations of Ub & Mtb shown at the bottom. A total of 100 bacterial cells counted. Two-tailed unpaired t-test performed. d Schematic representation of Mtb Rv1468c constructs used. e In vitro interaction analysis of Ub & GST-tagged full-length Mtb Rv1468c or the truncated mutants as indicated in d. Purified recombinant GST-tagged Mtb Rv1468c variants immobilized on glutathione sepharose resin. Mono-Ub (His6-tagged), K63 or K48-linked poly-Ub (Ub2–7) added, & then the protein complexes assayed by immunoblotting w/ indicated antibodies. f Immunoblot analysis of proteins immunoprecipitated (IP) w/ anti-Flag M2 Affinity Gel from lysates of HEK293T cells transfected w/ empty vector (–) or hemagglutinin (HA)-tagged Ub (+) & Flag-tagged wild-type (WT) Rv1468c or its mutants (V64G or L65G). g Confocal microscopy analysis of HeLa cells cotransfected w/ vectors encoding HA-tagged Ub (red) & GFP-tagged WT Rv1468c, V64G or L65G mutant (green). Nuclei (blue) stained w/ DNA-binding dye DAPI. Scale bars, 10 μm. Results representatives from at least 3 independent experiments (mean ± s.e.m. of n = 5 in c). The source data used in c provided in Source Data Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31036822), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-32867_mouse-monoclonal-hsp60-antibody-lk2-31020241612249.jpg)
![Western Blot: HSP60 Antibody (LK1) [NBP2-32973] - Western Blot Analysis of HeLa cell lysate using HSP60 Antibody (LK1)](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Western-Blot-NBP2-32973-img0010.jpg "Western Blot: HSP60 Antibody (LK1) [NBP2-32973] - Western Blot Analysis of HeLa cell lysate using HSP60 Antibody (LK1)")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (LK1) [NBP2-32973] - Immunofluorescent staining of PFA-fixed MCF-7 cells. followed by goat anti-Mouse IgG-CF488 (green). Nuclei are labeled with Red Dot (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Immunocytochemistry-Immunofluorescence-NBP2-32973-img0009.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (LK1) [NBP2-32973] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP60 MAb (LK1)](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Immunohistochemistry-Paraffin-NBP2-32973-img0001.jpg)
![Flow Cytometry: HSP60 Antibody (LK1) [NBP2-32973] - Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells using HSP60 antibody (LK1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Flow-Cytometry-NBP2-32973-img0011.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (LK1) [NBP2-32973] - Formalin-fixed paraffin-embedded human pancreas stained with HSP60 Monoclonal Antibody (LK1).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Immunohistochemistry-Paraffin-NBP2-32973-img0005.jpg)
![Simple Western: HSP60 Antibody (LK1) [NBP2-32973] - Simple Western lane view shows a specific band for HSP60 in 0.2 mg/ml of HeLa (left) and HepG2 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Simple-Western-NBP2-32973-img0002.jpg)
![Simple Western: HSP60 Antibody (LK1) [NBP2-32973] - Electropherogram image of the corresponding Simple Western lane. HSP60 antibody was used at 10 ug/ml dilution of HeLa and HepG2 lysates(s) respectively.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Simple-Western-NBP2-32973-img0004.jpg)
![Western Blot: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Western Blot analysis of HeLa cell lysate using HSP60 Antibody (HSPD1/780)](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Western-Blot-NBP2-44758-img0008.jpg "Western Blot: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Western Blot analysis of HeLa cell lysate using HSP60 Antibody (HSPD1/780)")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Immunofluorescent staining of PFA-fixed MCF-7 cells. HSP60 Antibody (HSPD1/780) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Red Dot (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Immunocytochemistry-Immunofluorescence-NBP2-44758-img0006.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Human Renal Cell Carcinoma stained with HSP60 Monoclonal Antibody (HSPD1/780)](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Immunohistochemistry-Paraffin-NBP2-44758-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Immunofluorescent staining of MeOH-fixed HeLa cells. HSP60 Antibody (HSPD1/780) followed by goat anti-Mouse IgG-CF488 (green). Nuclei are labeled with Red Dot (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Immunocytochemistry-Immunofluorescence-NBP2-44758-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Human Bladder Carcinoma stained with HSP60 Monoclonal Antibody (HSPD1/780)](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Immunohistochemistry-Paraffin-NBP2-44758-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Human Lung Carcinoma stained with HSP60 Monoclonal Antibody (HSPD1/780)](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Immunohistochemistry-Paraffin-NBP2-44758-img0002.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Human Pancreas stained with HSP60 Monoclonal Antibody (HSPD1/780).](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Immunohistochemistry-Paraffin-NBP2-44758-img0003.jpg)
![Protein Array: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HSP60 Antibody (HSPD1/780) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Protein-Array-NBP2-44758-img0007.jpg)
![Western Blot: HSP60 Antibody (3A2) [NBP2-46008] - Analysis of extracts (35ug) from 9 different cell lines by using HSPD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/HSP60-Antibody-3A2-Western-Blot-NBP2-46008-img0014.jpg "Western Blot: HSP60 Antibody (3A2) [NBP2-46008] - Analysis of extracts (35ug) from 9 different cell lines by using HSPD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: HSP60 Antibody (3A2) [NBP2-46008] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HSP60-Antibody-3A2-Immunohistochemistry-NBP2-46008-img0013.jpg)
![Western Blot: HSP60 Antibody (3A2) [NBP2-46008] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSP60.](http://images.novusbio.com/fullsize/HSP60-Antibody-3A2-Western-Blot-NBP2-46008-img0015.jpg)
![Immunohistochemistry: HSP60 Antibody (3A2) [NBP2-46008] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HSP60-Antibody-3A2-Immunohistochemistry-NBP2-46008-img0001.jpg)
![Immunohistochemistry: HSP60 Antibody (3A2) [NBP2-46008] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HSP60-Antibody-3A2-Immunohistochemistry-NBP2-46008-img0002.jpg)
![Immunohistochemistry: HSP60 Antibody (3A2) [NBP2-46008] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HSP60-Antibody-3A2-Immunohistochemistry-NBP2-46008-img0003.jpg)
![Immunohistochemistry: HSP60 Antibody (3A2) [NBP2-46008] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HSP60-Antibody-3A2-Immunohistochemistry-NBP2-46008-img0004.jpg)
![Immunohistochemistry: HSP60 Antibody (3A2) [NBP2-46008] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HSP60-Antibody-3A2-Immunohistochemistry-NBP2-46008-img0005.jpg)
![Immunohistochemistry: HSP60 Antibody (3A2) [NBP2-46008] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HSP60-Antibody-3A2-Immunohistochemistry-NBP2-46008-img0006.jpg)
![Immunohistochemistry: HSP60 Antibody (3A2) [NBP2-46008] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HSP60-Antibody-3A2-Immunohistochemistry-NBP2-46008-img0007.jpg)
![Immunohistochemistry: HSP60 Antibody (3A2) [NBP2-46008] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HSP60-Antibody-3A2-Immunohistochemistry-NBP2-46008-img0008.jpg)
![Immunohistochemistry: HSP60 Antibody (3A2) [NBP2-46008] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HSP60-Antibody-3A2-Immunohistochemistry-NBP2-46008-img0009.jpg)
![Immunohistochemistry: HSP60 Antibody (3A2) [NBP2-46008] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HSP60-Antibody-3A2-Immunohistochemistry-NBP2-46008-img0010.jpg)
![Immunohistochemistry: HSP60 Antibody (3A2) [NBP2-46008] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HSP60-Antibody-3A2-Immunohistochemistry-NBP2-46008-img0011.jpg)
![Immunohistochemistry: HSP60 Antibody (3A2) [NBP2-46008] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HSP60-Antibody-3A2-Immunohistochemistry-NBP2-46008-img0012.jpg)
![Western Blot: HSP60 Antibody (3G8) [NBP2-37617] - Western blot analysis using HSP60 mouse mAb against T47D (1), Hela (2), HepG2 (3), A549 (4), Jurkat (5), HEK293 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.](http://images.novusbio.com/fullsize/HSP60-Antibody-3G8-Western-Blot-NBP2-37617-img0002.jpg "Western Blot: HSP60 Antibody (3G8) [NBP2-37617] - Western blot analysis using HSP60 mouse mAb against T47D (1), Hela (2), HepG2 (3), A549 (4), Jurkat (5), HEK293 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (3G8) [NBP2-37617] - Immunofluorescence analysis of 3T3-L1 cells using HSP60 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/HSP60-Antibody-3G8-Immunocytochemistry-Immunofluorescence-NBP2-37617-img0003.jpg)
![Immunohistochemistry: HSP60 Antibody (3G8) [NBP2-37617] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and colon cancer tissues (right) using HSP60 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HSP60-Antibody-3G8-Immunohistochemistry-NBP2-37617-img0006.jpg)
![Flow Cytometry: HSP60 Antibody (3G8) [NBP2-37617] - Flow cytometric analysis of Hela cells using HSP60 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/HSP60-Antibody-3G8-Flow-Cytometry-NBP2-37617-img0004.jpg)
![Immunohistochemistry: HSP60 Antibody (3G8) [NBP2-37617] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using HSP60 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HSP60-Antibody-3G8-Immunohistochemistry-NBP2-37617-img0005.jpg)
![ELISA: HSP60 Antibody (3G8) [NBP2-37617] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/HSP60-Antibody-3G8-ELISA-NBP2-37617-img0001.jpg)
![Western Blot: HSP60 Antibody (SPM253) [NBP2-32866] - Western Blot Analysis of HeLa cell lysate using HSP60 Antibody (SPM253)](http://images.novusbio.com/fullsize/HSP60-Antibody-SPM253-Western-Blot-NBP2-32866-img0005.jpg "Western Blot: HSP60 Antibody (SPM253) [NBP2-32866] - Western Blot Analysis of HeLa cell lysate using HSP60 Antibody (SPM253)")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (SPM253) [NBP2-32866] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells, followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).](http://images.novusbio.com/fullsize/HSP60-Antibody-SPM253-Immunocytochemistry-Immunofluorescence-NBP2-32866-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (SPM253) [NBP2-32866] - Formalin-fixed, paraffin-embedded human pancreas stained with HSP60 Monoclonal Antibody (SPM253)](http://images.novusbio.com/fullsize/HSP60-Antibody-SPM253-Immunohistochemistry-Paraffin-NBP2-32866-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (SPM253) [NBP2-32866] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with HSP60 Monoclonal Antibody (SPM253)](http://images.novusbio.com/fullsize/HSP60-Antibody-SPM253-Immunohistochemistry-Paraffin-NBP2-32866-img0002.jpg)
![Western Blot: HSP60 Antibody [NBP1-89730] - Analysis using Anti-HSPD1 antibody NBP1-89730 (A) shows similar pattern to independent antibody NBP2-55503 (B).](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP1-89730-img0013.jpg "<b>Independent Antibodies Validation. </b>Western Blot: HSP60 Antibody [NBP1-89730] - Analysis using Anti-HSPD1 antibody NBP1-89730 (A) shows similar pattern to independent antibody NBP2-55503 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-89730] - Staining of human cell line U-251 MG shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89730-img0012.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody [NBP1-89730] - Staining of human kidney shows strong cytoplasmic positivity in cells of renal tubules.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunohistochemistry-Paraffin-NBP1-89730-img0008.jpg)
![Western Blot: HSP60 Antibody [NBP1-89730] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP1-89730-img0010.jpg)
![Western Blot: HSP60 Antibody [NBP1-89730] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP1-89730-img0011.jpg)
![Western Blot: HSP60 Antibody (GROEL/730) [NBP2-32868] - Western Blot Analysis of HeLa cell lysate using HSP60 Antibody (GROEL/730).](http://images.novusbio.com/fullsize/HSP60-Antibody-GROEL-730-Western-Blot-NBP2-32868-img0005.jpg "Western Blot: HSP60 Antibody (GROEL/730) [NBP2-32868] - Western Blot Analysis of HeLa cell lysate using HSP60 Antibody (GROEL/730).")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (GROEL/730) [NBP2-32868] - Confocal immunofluorescence image of HeLa cells using HSP60 Antibody (GROEL/730). Green (CF488) and Red Dot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/HSP60-Antibody-GROEL-730-Immunocytochemistry-Immunofluorescence-NBP2-32868-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (GROEL/730) [NBP2-32868] - Formalin-fixed, paraffin-embedded human Pancreas stained with HSP60 Monoclonal Antibody (GROEL/730).](http://images.novusbio.com/fullsize/HSP60-Antibody-GROEL-730-Immunohistochemistry-Paraffin-NBP2-32868-img0003.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (GROEL/730) [NBP2-32868] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with HSP60 Monoclonal Antibody (GROEL/730)](http://images.novusbio.com/fullsize/HSP60-Antibody-GROEL-730-Immunohistochemistry-Paraffin-NBP2-32868-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (GROEL/730) [NBP2-32868] - Formalin-fixed, paraffin-embedded human Tonsil stained with HSP60 Monoclonal Antibody (GROEL/730).](http://images.novusbio.com/fullsize/HSP60-Antibody-GROEL-730-Immunohistochemistry-Paraffin-NBP2-32868-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (HSPD1/875) [NBP2-44759] - Confocal immunofluorescence image of MeOH-fixed Hela cells using HSP60 antibody (HSPD1/875) followed by goat anti-mouse IgG-CF488 (green).Nuclei stained with RedDot.](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-875-Immunocytochemistry-Immunofluorescence-NBP2-44759-img0004.jpg "Immunocytochemistry/Immunofluorescence: HSP60 Antibody (HSPD1/875) [NBP2-44759] - Confocal immunofluorescence image of MeOH-fixed Hela cells using HSP60 antibody (HSPD1/875) followed by goat anti-mouse IgG-CF488 (green).Nuclei stained with RedDot.")
![Immunohistochemistry-Paraffin: HSP60 Antibody (HSPD1/875) [NBP2-44759] - Formalin-fixed, paraffin-embedded human liver in colon stained with HSP60 Mouse Monoclonal Antibody (HSPD1/875).](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-875-Immunohistochemistry-Paraffin-NBP2-44759-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (HSPD1/875) [NBP2-44759] - Confocal immunofluorescence image of HeLa cells using HSP60 Antibody (HSPD1/875) Green (CF488) and Red Dot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-875-Immunocytochemistry-Immunofluorescence-NBP2-44759-img0001.jpg)
![Protein Array: HSP60 Antibody (HSPD1/875) [NBP2-44759] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HSP60 Antibody (HSPD1/875). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-875-Protein-Array-NBP2-44759-img0002.jpg)
![Western Blot: HSP60 Antibody [NBP2-12734] - Western blot analysis of Parasite Lysates showing detection of HSP60 protein using Rabbit Anti-HSP60 Polyclonal Antibody (NBP2-12734). Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody (NBP2-12734) at 1:1000.](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP2-12734-img0003.jpg "Western Blot: HSP60 Antibody [NBP2-12734] - Western blot analysis of Parasite Lysates showing detection of HSP60 protein using Rabbit Anti-HSP60 Polyclonal Antibody (NBP2-12734). Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody (NBP2-12734) at 1:1000.")
![Immunohistochemistry: HSP60 Antibody [NBP2-12734] - Immunohistochemistry analysis using Rabbit Anti-HSP60 Polyclonal Antibody (NBP2-12734). Tissue: Liver. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody (NBP2-12734).](http://images.novusbio.com/fullsize/HSP60 Antibody-Immunohistochemistry-NBP2-12734-img0004.jpg)
![Western Blot: HSP60 Antibody [NBP2-12734] - Analysis of Parasite Lysates showing detection of HSP60 protein using Rabbit Anti-HSP60 Polyclonal Antibody . Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP2-12734-img0002.jpg)