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![SDS-Page: Recombinant Human TATA binding protein TBP Protein [H00006908-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00006908-Q01-1.jpg "SDS-Page: Recombinant Human TATA binding protein TBP Protein [H00006908-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: TATA binding protein TBP Antibody [NBP3-13174] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TATA binding protein TBP antibody (NBP3-13174) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TATA-binding-protein-TBP-Antibody-Western-Blot-NBP3-13174-img0001.jpg "Western Blot: TATA binding protein TBP Antibody [NBP3-13174] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TATA binding protein TBP antibody (NBP3-13174) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: TATA binding protein TBP Antibody [NBP3-13174] - TATA binding protein TBP antibody detects TATA binding protein TBP protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. TATA binding protein TBP stained by TATA binding protein TBP antibody (NBP3-13174) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TATA-binding-protein-TBP-Antibody-Immunohistochemistry-Paraffin-NBP3-13174-img0004.jpg)
![Immunohistochemistry-Paraffin: TATA binding protein TBP Antibody [NBP3-13174] - TATA binding protein TBP antibody detects TATA binding protein TBP protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. TATA binding protein TBP stained by TATA binding protein TBP antibody (NBP3-13174) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TATA-binding-protein-TBP-Antibody-Immunohistochemistry-Paraffin-NBP3-13174-img0002.jpg)
![Immunohistochemistry-Paraffin: TATA binding protein TBP Antibody [NBP3-13174] - TATA binding protein TBP antibody detects TATA binding protein TBP protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. TATA binding protein TBP stained by TATA binding protein TBP antibody (NBP3-13174) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TATA-binding-protein-TBP-Antibody-Immunohistochemistry-Paraffin-NBP3-13174-img0003.jpg)
![Western Blot: TATA binding protein TBP Antibody (C.15200002) [NBP2-59208] - Whole cell extracts (40 ug) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against TBP diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/TATA-binding-protein-TBP-Antibody-C-15200002-Western-Blot-NBP2-59208-img0001.jpg "Western Blot: TATA binding protein TBP Antibody (C.15200002) [NBP2-59208] - Whole cell extracts (40 ug) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against TBP diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation Sequencing: TATA binding protein TBP Antibody (C.15200002) [NBP2-59208] - IP'd DNA was analyzed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (A). The IP'd DNA was analyzed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.](http://images.novusbio.com/fullsize/TATA-binding-protein-TBP-Antibody-C-15200002-Chromatin-Immunoprecipitation-Sequencing-NBP2-59208-img0005.jpg)
![Chromatin Immunoprecipitation: TATA binding protein TBP Antibody (C.15200002) [NBP2-59208] - ChIP results obtained with the antibody against TBP. ChIP assays were performed using U2OS cells, the antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes, a region 0.5 and 1 kb upstream of the GAPDH promoter, respectively, and for exon 2 of the myoglobin gene as a negative control. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.](http://images.novusbio.com/fullsize/TATA-binding-protein-TBP-Antibody-C-15200002-Chromatin-Immunoprecipitation-NBP2-59208-img0002.jpg)
![Chromatin Immunoprecipitation: TATA binding protein TBP Antibody (C.15200002) [NBP2-59208] - ChIP was performed with 5 ug antibody on sheared chromatin from 1 million HeLaS3 cells using a Histone ChIP-seq kit.](http://images.novusbio.com/fullsize/TATA-binding-protein-TBP-Antibody-C-15200002-Chromatin-Immunoprecipitation-NBP2-59208-img0006.jpg)
![Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - Staining of Pig Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence](http://images.novusbio.com/fullsize/TATA-binding-protein-TBP-Antibody-Western-Blot-NBP2-12933-img0005.jpg "Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - Staining of Pig Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence")
![Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - NBP2-12933 (1ug/ml) staining of HeLa nuclear (A) and cytosolic (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TATA-binding-protein-TBP-Antibody-Western-Blot-NBP2-12933-img0002.jpg)
![Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - Analysis of cell line NIH3T3 (A), Mouse Testis (B) and Rat Testis (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence](http://images.novusbio.com/fullsize/TATA-binding-protein-TBP-Antibody-Western-Blot-NBP2-12933-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: TATA binding protein TBP Antibody [NBP2-38610] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/TATA-binding-protein-TBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38610-img0003.jpg "Immunocytochemistry/Immunofluorescence: TATA binding protein TBP Antibody [NBP2-38610] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.")
![Immunohistochemistry: TATA binding protein TBP Antibody [NBP2-38610] - Staining of human testis shows weak nuclear positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/TATA-binding-protein-TBP-Antibody-Immunohistochemistry-NBP2-38610-img0002.jpg)
![Western Blot: TATA binding protein TBP Antibody [NBP2-95183] - Analysis of extracts of Rat skeletal muscle, using TBP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/TATA-binding-protein-TBP-Antibody-Western-Blot-NBP2-95183-img0004.jpg "Western Blot: TATA binding protein TBP Antibody [NBP2-95183] - Analysis of extracts of Rat skeletal muscle, using TBP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Western Blot: TATA binding protein TBP Antibody [NBP3-02958] - Analysis of extracts of various cell lines, using TATA binding protein TBP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit](http://images.novusbio.com/fullsize/TATA-binding-protein-TBP-Antibody-Western-Blot-NBP3-02958-img0003.jpg "Western Blot: TATA binding protein TBP Antibody [NBP3-02958] - Analysis of extracts of various cell lines, using TATA binding protein TBP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit")
![Western Blot: TATA binding protein TBP Antibody (SR1605) [NBP3-21665] - Western blot analysis using NBP3-21665 in K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21665_rabbit-tata-binding-protein-tbp-mab-sr1605-57202315545111.jpg "Western Blot: TATA binding protein TBP Antibody (SR1605) [NBP3-21665] - Western blot analysis using NBP3-21665 in K562 cell lysate.")
![Western Blot: TATA binding protein TBP isoform 1 Antibody [NBP2-12932] - TATA binding protein TBP isoform 1 Antibody 0.3ug/ml staining of HeLa nuclear (A) and cytosolic (B) lysates (35ug protein in commercial extraction buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TATA-binding-protein-TBP-isoform-1-Antibody-Western-Blot-NBP2-12932-img0005.jpg "Western Blot: TATA binding protein TBP isoform 1 Antibody [NBP2-12932] - TATA binding protein TBP isoform 1 Antibody 0.3ug/ml staining of HeLa nuclear (A) and cytosolic (B) lysates (35ug protein in commercial extraction buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: TATA binding protein TBP Antibody (GT453) [NBP3-13596] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with TATA binding protein TBP antibody (NBP3-13596) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TATA-binding-protein-TBP-Antibody-GT453-Western-Blot-NBP3-13596-img0001.jpg "Western Blot: TATA binding protein TBP Antibody (GT453) [NBP3-13596] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with TATA binding protein TBP antibody (NBP3-13596) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")