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![Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042] - Staining in human testis and liver tissues using anti-CCT5 antibody. Corresponding CCT5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CCT5-Antibody-Immunohistochemistry-Paraffin-NBP1-83042-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042] - Staining in human testis and liver tissues using anti-CCT5 antibody. Corresponding CCT5 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: CCT5 Antibody [NBP1-83042] - Analysis using Anti-CCT5 antibody NBP1-83042 (A) shows similar pattern to independent antibody NBP1-83043 (B).](http://images.novusbio.com/fullsize/CCT5-Antibody-Western-Blot-NBP1-83042-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042] - Staining of human cerebral cortex, colon, liver and testis using Anti-CCT5 antibody NBP1-83042 (A) shows similar protein distribution across tissues to independent antibody NBP1-83043 (B).](http://images.novusbio.com/fullsize/CCT5-Antibody-Immunohistochemistry-Paraffin-NBP1-83042-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: CCT5 Antibody [NBP1-83042] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytosol.](http://images.novusbio.com/fullsize/CCT5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83042-img0007.jpg)
![Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/CCT5-Antibody-Immunohistochemistry-Paraffin-NBP1-83042-img0014.jpg)
![Western Blot: CCT5 Antibody [NBP1-83042] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/CCT5-Antibody-Western-Blot-NBP1-83042-img0003.jpg)
![Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/CCT5-Antibody-Immunohistochemistry-Paraffin-NBP1-83042-img0008.jpg)
![Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/CCT5-Antibody-Immunohistochemistry-Paraffin-NBP1-83042-img0009.jpg)
![Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042] - Staining of human colon.](http://images.novusbio.com/fullsize/CCT5-Antibody-Immunohistochemistry-Paraffin-NBP1-83042-img0013.jpg)
![Immunohistochemistry-Paraffin: CCT8 Antibody [NBP1-88285] - Staining of human cerebral cortex, lymph node, pancreas and testis using Anti-CCT8 antibody NBP1-88285 (A) shows similar protein distribution across tissues to independent antibody NBP1-88287 (B).](http://images.novusbio.com/fullsize/CCT8-Antibody-Immunohistochemistry-Paraffin-NBP1-88285-img0012.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CCT8 Antibody [NBP1-88285] - Staining of human cerebral cortex, lymph node, pancreas and testis using Anti-CCT8 antibody NBP1-88285 (A) shows similar protein distribution across tissues to independent antibody NBP1-88287 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: CCT8 Antibody [NBP1-88285] - Analysis using Anti-CCT8 antibody NBP1-88285 (A) shows similar pattern to independent antibody NBP1-88287 (B).](http://images.novusbio.com/fullsize/CCT8-Antibody-Western-Blot-NBP1-88285-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CCT8 Antibody [NBP1-88285] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & intermediate filaments.](http://images.novusbio.com/fullsize/CCT8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88285-img0008.jpg)
![Immunohistochemistry-Paraffin: CCT8 Antibody [NBP1-88285] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/CCT8-Antibody-Immunohistochemistry-Paraffin-NBP1-88285-img0017.jpg)
![Western Blot: CCT8 Antibody [NBP1-88285] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/CCT8-Antibody-Western-Blot-NBP1-88285-img0006.jpg)
![Immunohistochemistry-Paraffin: CCT8 Antibody [NBP1-88285] - Staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/CCT8-Antibody-Immunohistochemistry-Paraffin-NBP1-88285-img0014.jpg)
![Immunohistochemistry-Paraffin: CCT8 Antibody [NBP1-88285] - Staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/CCT8-Antibody-Immunohistochemistry-Paraffin-NBP1-88285-img0015.jpg)
![Immunohistochemistry-Paraffin: CCT8 Antibody [NBP1-88285] - Staining of human testis shows strong cytoplasmic positivity in seminiferous ducts.](http://images.novusbio.com/fullsize/CCT8-Antibody-Immunohistochemistry-Paraffin-NBP1-88285-img0016.jpg)
![Western Blot: CCT5 Antibody (4E5-4B1) [H00022948-M01] - CCT5 monoclonal antibody (M01), clone 4E5-4B1 Analysis of CCT5 expression in Hela.](http://images.novusbio.com/fullsize/CCT5-Antibody-4E5-4B1-Western-Blot-H00022948-M01-img0010.jpg "Western Blot: CCT5 Antibody (4E5-4B1) [H00022948-M01] - CCT5 monoclonal antibody (M01), clone 4E5-4B1 Analysis of CCT5 expression in Hela.")
![Immunocytochemistry/Immunofluorescence: CCT5 Antibody (4E5-4B1) [H00022948-M01] - Analysis of monoclonal antibody to CCT5 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CCT5-Antibody-4E5-4B1-Immunocytochemistry-Immunofluorescence-H00022948-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: CCT5 Antibody (4E5-4B1) [H00022948-M01] - Analysis of monoclonal antibody to CCT5 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/CCT5-Antibody-4E5-4B1-Immunohistochemistry-Paraffin-H00022948-M01-img0008.jpg)
![ELISA: CCT5 Antibody (4E5-4B1) [H00022948-M01] - Detection limit for recombinant GST tagged CCT5 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CCT5-Antibody-4E5-4B1-Sandwich-ELISA-H00022948-M01-img0009.jpg)
![Western Blot: CCT8 Antibody [NBP1-88287] - Analysis using Anti-CCT8 antibody NBP1-88287 (A) shows similar pattern to independent antibody NBP1-88286 (B).](http://images.novusbio.com/fullsize/CCT8-Antibody-Western-Blot-NBP1-88287-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CCT8 Antibody [NBP1-88287] - Analysis using Anti-CCT8 antibody NBP1-88287 (A) shows similar pattern to independent antibody NBP1-88286 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CCT8 Antibody [NBP1-88287] - Staining of human lymph node.](http://images.novusbio.com/fullsize/CCT8-Antibody-Immunohistochemistry-Paraffin-NBP1-88287-img0012.jpg)
![Western Blot: CCT8 Antibody [NBP1-88287] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/CCT8-Antibody-Western-Blot-NBP1-88287-img0005.jpg)
![Immunohistochemistry-Paraffin: CCT8 Antibody [NBP1-88287] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/CCT8-Antibody-Immunohistochemistry-Paraffin-NBP1-88287-img0001.jpg)
![Immunohistochemistry-Paraffin: CCT8 Antibody [NBP1-88287] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize/CCT8-Antibody-Immunohistochemistry-Paraffin-NBP1-88287-img0006.jpg)
![Immunohistochemistry-Paraffin: CCT8 Antibody [NBP1-88287] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/CCT8-Antibody-Immunohistochemistry-Paraffin-NBP1-88287-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CCT8 Antibody [NBP1-88287] - Staining in human adrenal gland and pancreas tissues using anti-CCT8 antibody. Corresponding CCT8 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CCT8-Antibody-Immunohistochemistry-Paraffin-NBP1-88287-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CCT8 Antibody [NBP1-88287] - Staining of human cerebral cortex, lymph node, pancreas and testis using Anti-CCT8 antibody NBP1-88287 (A) shows similar protein distribution across tissues to independent antibody NBP1-88285 (B).](http://images.novusbio.com/fullsize/CCT8-Antibody-Immunohistochemistry-Paraffin-NBP1-88287-img0009.jpg)
![Immunohistochemistry-Paraffin: CCT8 Antibody [NBP1-88287] - Staining of human testis.](http://images.novusbio.com/fullsize/CCT8-Antibody-Immunohistochemistry-Paraffin-NBP1-88287-img0011.jpg)
![Immunohistochemistry-Paraffin: CCT8 Antibody [NBP1-88287] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/CCT8-Antibody-Immunohistochemistry-Paraffin-NBP1-88287-img0013.jpg)
![Western Blot: CCT5 Antibody [NBP1-83043] - Analysis using Anti-CCT5 antibody NBP1-83043 (A) shows similar pattern to independent antibody NBP1-83042 (B).](http://images.novusbio.com/fullsize/CCT5-Antibody-Western-Blot-NBP1-83043-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CCT5 Antibody [NBP1-83043] - Analysis using Anti-CCT5 antibody NBP1-83043 (A) shows similar pattern to independent antibody NBP1-83042 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83043] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/CCT5-Antibody-Immunohistochemistry-Paraffin-NBP1-83043-img0010.jpg)
![Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83043] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/CCT5-Antibody-Immunohistochemistry-Paraffin-NBP1-83043-img0005.jpg)
![Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83043] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/CCT5-Antibody-Immunohistochemistry-Paraffin-NBP1-83043-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83043] - Staining in human testis and liver tissues using anti-CCT5 antibody. Corresponding CCT5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CCT5-Antibody-Immunohistochemistry-Paraffin-NBP1-83043-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83043] - Staining of human cerebral cortex, colon, liver and testis using Anti-CCT5 antibody NBP1-83043 (A) shows similar protein distribution across tissues to independent antibody NBP1-83042 (B).](http://images.novusbio.com/fullsize/CCT5-Antibody-Immunohistochemistry-Paraffin-NBP1-83043-img0008.jpg)
![Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83043] - Staining of human colon.](http://images.novusbio.com/fullsize/CCT5-Antibody-Immunohistochemistry-Paraffin-NBP1-83043-img0011.jpg)
![Western Blot: CCT3 Antibody [NBP1-86562] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-CCT3 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/CCT3-Antibody-Western-Blot-NBP1-86562-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CCT3 Antibody [NBP1-86562] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-CCT3 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CCT3 Antibody [NBP1-86562] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CCT3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86562-img0011.jpg)
![Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-86562] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CCT3-Antibody-Immunohistochemistry-Paraffin-NBP1-86562-img0010.jpg)
![Western Blot: CCT3 Antibody [NBP1-86562] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/CCT3-Antibody-Western-Blot-NBP1-86562-img0008.jpg)
![Western Blot: CCT5 Antibody [NBP1-31337] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [N1N3].](http://images.novusbio.com/fullsize/CCT5-Antibody-Western-Blot-NBP1-31337-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CCT5 Antibody [NBP1-31337] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [N1N3].")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CCT5 Antibody [NBP1-31337] - Analysis of HeLa, using NBP1-31337 at 1:200 dilution.](http://images.novusbio.com/fullsize/CCT5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31337-img0002.jpg)
![Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-31337] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CCT5-Antibody-Immunohistochemistry-Paraffin-NBP1-31337-img0003.jpg)
![Western Blot: CCT5 Antibody [NBP1-31337] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CCT5-Antibody-Western-Blot-NBP1-31337-img0004.jpg)
![Immunohistochemistry: CCT5 Antibody [NBP1-31337] - Immunohistochemical analysis of frozen sections of zebrafish muscle, 5 hpf, using CCT5 antibody [N1N3] (NBP1-31337) at 1:100 dilution.](http://images.novusbio.com/fullsize/nbp1-31337_rabbit-polyclonal-cct5-antibody-91020242013126.jpg)
![Western Blot: CCT5 Antibody (639) [NBP2-43680] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CCT5 Antibody (639) diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CCT5-Antibody-639-Western-Blot-NBP2-43680-img0007.jpg "Western Blot: CCT5 Antibody (639) [NBP2-43680] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CCT5 Antibody (639) diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: CCT5 Antibody (639) [NBP2-43680] - CCT5 Antibody (639) detects CCT5 protein at centrosome and cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CCT5 stained by CCT5 Antibody (639) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/CCT5-Antibody-639-Immunocytochemistry-Immunofluorescence-NBP2-43680-img0006.jpg)
![Western Blot: CCT5 Antibody (639) [NBP2-43680] - Analysis of various whole cell extracts (30 ug) were separated by 7.5 % SDS-PAGE, and blotted with TCP1 epsilon antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/CCT5-Antibody-639-Western-Blot-NBP2-43680-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CCT5 Antibody (639) [NBP2-43680] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [639].](http://images.novusbio.com/fullsize/CCT5-Antibody-639-Western-Blot-NBP2-43680-img0005.jpg)
![Immunoprecipitation: CCT5 Antibody (639) [NBP2-43680] - Analysis of TCP1 epsilon protein from 293T whole cell extracts using 5 ug of TCP1 epsilon antibody [639]. Western blot analysis was performed using TCP1 epsilon antibody [639]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/CCT5-Antibody-639-Immunoprecipitation-NBP2-43680-img0003.jpg)
![Western Blot: CCT8 Antibody [NBP1-88286] - Analysis using Anti-CCT8 antibody NBP1-88286 (A) shows similar pattern to independent antibody NBP1-88287 (B).](http://images.novusbio.com/fullsize/CCT8-Antibody-Western-Blot-NBP1-88286-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CCT8 Antibody [NBP1-88286] - Analysis using Anti-CCT8 antibody NBP1-88286 (A) shows similar pattern to independent antibody NBP1-88287 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: CCT8 Antibody [NBP1-88286] - Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.](http://images.novusbio.com/fullsize/CCT8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88286-img0006.jpg)
![Immunohistochemistry-Paraffin: CCT8 Antibody [NBP1-88286] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CCT8-Antibody-Immunohistochemistry-Paraffin-NBP1-88286-img0007.jpg)
![Western Blot: CCT8 Antibody [NBP1-88286] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/CCT8-Antibody-Western-Blot-NBP1-88286-img0005.jpg)
![Western Blot: CCT3 Antibody [NBP1-79040] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CCT3 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCT3 was also immunoprecipitated by rabbit anti-CCT3 antibody NBP1-79041 which recognizes a downstream epitope](http://images.novusbio.com/fullsize/CCT3-Antibody-Western-Blot-NBP1-79040-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CCT3 Antibody [NBP1-79040] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CCT3 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCT3 was also immunoprecipitated by rabbit anti-CCT3 antibody NBP1-79041 which recognizes a downstream epitope")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-79040] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti- CCT3 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/CCT3-Antibody-Immunohistochemistry-NBP1-79040-img0007.jpg)
![Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-79040] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- CCT3 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/CCT3-Antibody-Immunohistochemistry-NBP1-79040-img0006.jpg)
![Western Blot: CCT3 Antibody [NBP1-79041] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CCT3 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCT3 was also immunoprecipitated by rabbit anti-CCT3 antibody NBP1-79040 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/CCT3-Antibody-Western-Blot-NBP1-79041-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CCT3 Antibody [NBP1-79041] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CCT3 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCT3 was also immunoprecipitated by rabbit anti-CCT3 antibody NBP1-79040 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-79041] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- CCT3 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/CCT3-Antibody-Immunohistochemistry-NBP1-79041-img0009.jpg)
![Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-79041] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- CCT3 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/CCT3-Antibody-Immunohistochemistry-NBP1-79041-img0008.jpg)
![Immunohistochemistry-Paraffin: CCT8 Antibody [NBP1-33486] - TCP1 theta antibody detects TCP1 theta protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TCP1 theta antibody diluted at 1:500.](http://images.novusbio.com/fullsize/CCT8-Antibody-Immunohistochemistry-Paraffin-NBP1-33486-img0006.jpg "Immunohistochemistry-Paraffin: CCT8 Antibody [NBP1-33486] - TCP1 theta antibody detects TCP1 theta protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TCP1 theta antibody diluted at 1:500.")
![Western Blot: CCT8 Antibody [NBP1-33486] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/CCT8-Antibody-Western-Blot-NBP1-33486-img0004.jpg)
![Immunohistochemistry-Paraffin: CCT8 Antibody [NBP1-33486] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CCT8-Antibody-Immunohistochemistry-Paraffin-NBP1-33486-img0003.jpg)
![Western Blot: CCT8 Antibody [NBP1-33486] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CCT8 antibody [N2C2], Internal (NBP1-33486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33486_rabbit-polyclonal-cct8-antibody-91020242091447.jpg)