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![Simple Western: SR-BI Antibody [NB400-104] - Image shows a specific band for SR-BI in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Simple-Western-NB400-104-img0014.jpg "Simple Western: SR-BI Antibody [NB400-104] - Image shows a specific band for SR-BI in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human adrenal cell lysate.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Western-Blot-NB400-104-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human fibroblast samples fixed in 4% PFA and permeabilized in PBS (0.2% Tween). Primary incubation overnight at 4C using a 1:100 dilution in PBS (0.1% Tween) with 1% BSA. Secondary antibody is anti-rabbit conjugate to Alexa Fluor 488. SR-BI is shown in green and nucleus in blue (Hoescht 33342 stain).](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunocytochemistry-Immunofluorescence-NB400-104-img0030.jpg)
![Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on HeLa cells with SR-BI antibody NB400-104AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Flow-Cytometry-NB400-104-img0026.jpg)
![Western Blot: SR-BI Antibody [NB400-104] - Detection of SR-BI in rat H4IIE total cell lysates and plasma membrane proteins. Photo courtesy of product review by verified customer.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Western-Blot-NB400-104-img0013.jpg)
![Immunohistochemistry: SR-BI Antibody [NB400-104] - Immunolocalization of SR-BI in adult mink testis using NB400-104. SR-BI labeling is visible at the surface and along the outline of the large vacuole. Photo courtesy of R.M. Pelletier, University of Montreal.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunohistochemistry-NB400-104-img0011.jpg)
![Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - FFPE tissue section of mouse liver using SR-BI antibody (Lot 8310) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a specific membrane signal of SR-BI protein in the murine hepatocytes.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunohistochemistry-Paraffin-NB400-104-img0022.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: SR-BI Antibody [NB400-104] - HCV replication was increased by CD36. Silence of CD36 decreased HCV infection and replication (n = 3). Huh7.5 cells were transfected with 0.2 u g CD36-HA plasmid or with 150 pmol siRNA for CD36 (B) for 48 hrs, and then were washed and infected with HCV (150 IU/cell) for 2 hrs, followed by washing and continuously incubating. Intracellular HCV RNA and proteins were detected in 96 hrs, and the cytotoxicity of siRNAs was measured in 96-wells plate with a MTT assay in B. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21808) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Western-Blot-NB400-104-img0034.jpg)
![Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - Staining of human adrenal cortex.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunohistochemistry-Paraffin-NB400-104-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: SR-BI Antibody [NB400-104] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-SR-BI at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunocytochemistry-Immunofluorescence-NB400-104-img0029.jpg)
![Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-104] - FFPE tissue section of mouse liver using SR-BI antibody (Lot R-4) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated mainly a membranous signal of SR-BI protein in the murine hepatocytes.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Immunohistochemistry-Paraffin-NB400-104-img0023.jpg)
![Flow Cytometry: SR-BI Antibody [NB400-104] - Analysis of Huh7 and HepG2 cells using SR-B1 antibody NB400-104. Courtesy of Bruno Sainz, Jr., PhD, University of Illinois at Chicago.](http://images.novusbio.com/fullsize/SR-BI-Antibody-Flow-Cytometry-NB400-104-img0004.jpg)
![Western Blot: SR-BI Antibody - BSA Free [NB400-104] - Effects of 13-HODE & LA in the presence & absence of PPAR alpha & PPAR gamma selective antagonists on molecular markers of cholesterol homeostasis in RAW264.7 macrophages. RAW264.7 cells were pre-treated without or with the PPAR alpha selective antagonist GW6471 or the PPAR gamma selective antagonist GW9662 & subsequently treated without (vehicle control) or with 2.5 μmol/L 13-HODE or 100 μmol/L LA for 24 h. Afterwards, cells were lysed & subsequently processed for western blotting as described in the materials & methods section. A, Representative immunoblots specific for ABCA1, ABCG1, SR-BI, LXR alpha , & beta -actin which was used for normalization are shown. B, Bars represent data from densitometric analysis & are means ± SD from three independent experiments (n = 3). Data are expressed as percentage of protein concentration of vehicle control cells. Results from statistical analysis are indicated: Significant effects are denoted with superscript letters. Bars marked without a common superscript letter significantly differ (P 2000) (g, h, i, respectively). Bar graph shows the mean percentage of positively stained cells. Error bars depict standard deviations. ****p 0.3) by one-way ANOVA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29523748), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-104_rabbit-polyclonal-sr-bi-antibody-31020241618928.jpg)
![Western Blot: SR-BI Antibody - BSA Free [NB400-104] - Differentiation & characteristics of iHLCs. (a) Cell morphology & culture conditions at different stages of differentiation from iPSCs into iHLCs. Bright-field images were taken at the indicated time points (scale bar 100 µm). (b) Sequential expression & repression of transcription factors marking successful differentiation. Cells were immunostained with antibodies against Oct 3/4 (pluripotency marker), the endodermal transcription factor GATA-4, & the hepatic marker HNF4 alpha at day 0, 5, & 10 of differentiation (scale bars 20 µm). Shown are representative fluorescence & DIC images. (c) mRNA expression levels of different hepatocyte-specific factors in iHLCs, Huh7.5 cells, & primary human hepatocytes (PHHs) were analysed via qRT-PCR. Expression levels are displayed as fold over iPSCs normalised to GAPDH & 18S rRNA (mean ± SEM, n = 3–4, *p 0.9) by one-way ANOVA. §P < 0.02 by unpaired Student's t-test, relative to 0 h time point. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29523748), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-104_rabbit-polyclonal-sr-bi-antibody-31020241616010.jpg)
![Immunocytochemistry/Immunofluorescence: HEXIM2 Antibody [NBP1-93877] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/HEXIM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-93877-img0005.jpg "Immunocytochemistry/Immunofluorescence: HEXIM2 Antibody [NBP1-93877] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HEXIM2 Antibody [NBP1-93877] - Staining in human testis and heart muscle tissues using anti-HEXIM2 antibody. Corresponding HEXIM2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HEXIM2-Antibody-Immunohistochemistry-Paraffin-NBP1-93877-img0008.jpg)
![Immunohistochemistry-Paraffin: HEXIM2 Antibody [NBP1-93877] - Staining of human colon shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HEXIM2-Antibody-Immunohistochemistry-Paraffin-NBP1-93877-img0002.jpg)
![Immunohistochemistry-Paraffin: HEXIM2 Antibody [NBP1-93877] - Staining of human heart muscle shows low expression as expected.](http://images.novusbio.com/fullsize/HEXIM2-Antibody-Immunohistochemistry-Paraffin-NBP1-93877-img0006.jpg)
![Immunohistochemistry-Paraffin: HEXIM2 Antibody [NBP1-93877] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/HEXIM2-Antibody-Immunohistochemistry-Paraffin-NBP1-93877-img0007.jpg)
![Western Blot: HEXIM2 Antibody [NBP1-93876] - Analysis in control (vector only transfected HEK293T lysate) and HEXIM2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/HEXIM2-Antibody-Western-Blot-NBP1-93876-img0007.jpg "Western Blot: HEXIM2 Antibody [NBP1-93876] - Analysis in control (vector only transfected HEK293T lysate) and HEXIM2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HEXIM2 Antibody [NBP1-93876] - Staining in human testis and heart muscle tissues using anti-HEXIM2 antibody. Corresponding HEXIM2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HEXIM2-Antibody-Immunohistochemistry-Paraffin-NBP1-93876-img0006.jpg)
![Immunohistochemistry-Paraffin: HEXIM2 Antibody [NBP1-93876] - Staining of human heart muscle shows low expression as expected.](http://images.novusbio.com/fullsize/HEXIM2-Antibody-Immunohistochemistry-Paraffin-NBP1-93876-img0004.jpg)
![Immunohistochemistry-Paraffin: HEXIM2 Antibody [NBP1-93876] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/HEXIM2-Antibody-Immunohistochemistry-Paraffin-NBP1-93876-img0005.jpg)
![Western Blot: HEXIM2 Antibody [NBP2-16812] - Sample (30 ug of whole cell lysate) A: A549 B: HepG2 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/HEXIM2-Antibody-Western-Blot-NBP2-16812-img0001.jpg "Western Blot: HEXIM2 Antibody [NBP2-16812] - Sample (30 ug of whole cell lysate) A: A549 B: HepG2 12% SDS PAGE gel, diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: HEXIM2 Antibody [NBP2-16812] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HEXIM2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/HEXIM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16812-img0003.jpg)
![Immunohistochemistry-Paraffin: HEXIM2 Antibody [NBP2-16812] - Antibody detects HEXIM2 protein at nucleus on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. HEXIM2 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HEXIM2-Antibody-Immunohistochemistry-Paraffin-NBP2-16812-img0002.jpg)
![Western Blot: HEXIM2 Antibody [NBP3-04730] - Analysis of extracts of various cell lines, using HExIM2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit](http://images.novusbio.com/fullsize/HEXIM2-Antibody-Western-Blot-NBP3-04730-img0001.jpg "Western Blot: HEXIM2 Antibody [NBP3-04730] - Analysis of extracts of various cell lines, using HExIM2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit")
![Western Blot: HEXIM2 Overexpression Lysate (Adult Normal) [NBP2-04423] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HEXIM2.](http://images.novusbio.com/fullsize/HEXIM2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04423-img0001.jpg "Western Blot: HEXIM2 Overexpression Lysate (Adult Normal) [NBP2-04423] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HEXIM2.")
![Western Blot: Hexim 1 Antibody [NBP1-71867] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-HEXIM1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies NBP1-71866 and NBP1-71868, which recognize other epitopes.](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Western-Blot-NBP1-71867-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Hexim 1 Antibody [NBP1-71867] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-HEXIM1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies NBP1-71866 and NBP1-71868, which recognize other epitopes.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: Hexim 1 Antibody [NBP1-71867] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunohistochemistry-NBP1-71867-img0009.jpg)
![Immunohistochemistry: Hexim 1 Antibody [NBP1-71867] - Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml) ( ) or 1:1,000 (1ug/ml) ( and ). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunohistochemistry-NBP1-71867-img0008.jpg)
![Western Blot: Hexim 1 Antibody [NBP1-71868] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-HEXIM1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies NBP1-71866 and NBP1-71867, which recognize upstream epitopes.](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Western-Blot-NBP1-71868-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Hexim 1 Antibody [NBP1-71868] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-HEXIM1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies NBP1-71866 and NBP1-71867, which recognize upstream epitopes.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: Hexim 1 Antibody [NBP1-71868] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunohistochemistry-NBP1-71868-img0009.jpg)
![Immunohistochemistry: Hexim 1 Antibody [NBP1-71868] - Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml) ( ) or 1:1,000 (1ug/ml) ( and ). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunohistochemistry-NBP1-71868-img0008.jpg)