p62/SQSTM1 (sequestosome 1) is ubiquitously-expressed cytoplasmic/adaptor protein. SQSTM1 functions as a signaling hub for various signal transduction pathways involved in apoptosis, cell differentiation, apoptosis, immune response, and K+ channel regulation. It is conserved in vertebrates and can be induced by a wide variety of triggers including the proteasomal inhibitor PSI, PGJ2/prostaglandin J2, and the tumor promoting agent phorbol 12-myristate 13-acetate (PMA). The SQSTM1 protein regulates signaling cascades through ubiquitination, as it is essential for both the formation and autophagic degradation of polyubiquitin-containing bodies known as aggresome-like induced structures (ALIS).
Immunofluorescence: sequestosome 1 Antibody
The p62/SQSTM1 antibody has been used to show that the lack of Beclin1 cleavage by caspases contributes to cancer cell survival and therapeutic resistance1. Another cancer study looking at downstream-regulated genes in response to glucorticoids such as dexamethasone used the p62/SQSTM1 antibody to show these drugs apparently promote autophagy in T-lymphocytes2. Elegant studies with the p62/SQSTM1 antibody in murine models of aging found some interestingly novel effects on both endosomal proteostasis and the accumulation of oxidatively modified proteins in response to oxidative stress3. Neurodegenerative and dementia-disease state investigators have also relied on use of the p62/SQSTM1 antibody. Elrick et al relied upon the p62/SQSTM1 antibody to investigate the role of autophagosomes in the childhood-onset Niemann-Pick type C disease (NPC)4. Ohmi et al mapped brain pathological and histological patterns within mice afflicted with the childhood Sanfilippo syndrome type B (MPS IIIB) using the p62/SQSTM1 antibody5. Both groups were able to verify and validate the extent of autophagosome involvement in their afflicted samples.
Novus Biologicals offers various p62/SQSTM1 reagents for your research needs including:
