Fluorescent probes conjugated to antibodies allow for simultaneous IHC detection of multiple antigens in the same tissue section. However, quite often conventional multi-color IHC cannot be done if only primary antibodies raised in the same host species are available to the researcher. To solve this problem, we developed a novel technique for performing multicolor fluorescence immunohistochemistry using primary antibodies derived from a single host source. Alex Kalyuzhny, PhD, Immunohistochemistry and ELISpot Manager at Bio-Techne, will discuss modifications of primary antibodies allowing using fluorescent secondary antibodies so they bind only to primary antibodies of interest but don’t cross-react with irrelevant primary antibodies.
Register now for our FREE webinar on Thursday, January 14, 2016.
