TRPM8 is a transmembrane homo-tetramer ion channel that is activated by cold temperatures, cooling agents, and menthol stimuli. It belongs to a subgroup within the larger family of TRP cation channels (including the TRPV1 capsaicin receptor) that are named after the melastatin protein. These proteins are also known chanzymes due to their C-terminus enzyme domains. TRPM8 contains a voltage sensor as well as a binding domain for channel agonists such as menthol and icilin. Upon activation, it allows sodium and calcium entry, causing the creation of an action potential through depolarization. It is expressed in sensory neurons where it appears to play a role in neurite outgrowth, signaling, and apoptosis in response to noxious stimuli.
Immunohistochemistry: TRPV1 Antibody
Oddly enough, TRPM8 is expressed in areas such as the prostate, bladder, and lungs, where it serves a yet-to-be-identified role. The TRPM8 antibody is a rabbit polyclonal antibody and was used by Peabody’s group at NIH to monitor decision network on/off switches for Drosophila body development1. The TRPM8 activates N(CCAP), a small network of central neurons that then trigger all essential behavioral and somatic processes required for wing expansion. Ma’s group used the TRPM8 antibody in their brown adipose tissue (BAT) studies and found a novel role for TRPM8 in mediating BAT thermogenesis, a potentially promising treatment for obesity2.
Novus Biologicals offers TRPM8 reagents for your research needs including:
PMID
